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AMD 3465 hexahydrobromide

Name: AMD 3465 hexahydrobromide
Brand: TargetMol
SKU: T7208
Price: 31 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T7208Cas No. 185991-07-5

Alias GENZ-644494 (hexahydrobromide)

AMD 3465 hexahydrobromide (GENZ-644494 (hexahydrobromide)) is a CXCR4 receptor antagonist with potential anticancer and anti-HIV activity.

AMD 3465 hexahydrobromide

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Purity: 99.39%

Catalog No. T7208Alias GENZ-644494 (hexahydrobromide)Cas No. 185991-07-5

AMD 3465 hexahydrobromide (GENZ-644494 (hexahydrobromide)) is a CXCR4 receptor antagonist with potential anticancer and anti-HIV activity.

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$31	In Stock	In Stock
10 mg	$43	In Stock	In Stock
25 mg	$85	In Stock	In Stock
50 mg	$146	In Stock	In Stock
100 mg	$230	In Stock	In Stock
200 mg	$342	-	In Stock
1 mL x 10 mM (in DMSO)	$37	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:99.39%

Appearance:Solid

Color:White

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COA LCMS MS HNMR

Product Introduction

Bioactivity

Description	AMD 3465 hexahydrobromide (GENZ-644494 (hexahydrobromide)) is a CXCR4 receptor antagonist with potential anticancer and anti-HIV activity.
Targets&IC50	12G5 mAb-CXCR4:0.75 nM (IC50, in SupT1 cells), CXCL12 (AF647)-CXCR4:18 nM (IC50, in SupT1 cells), X4 HIV-1 (HE):9.8 nM (IC50, in MT-4 cells), X4 HIV-1 (NL4.3):6.1 nM (IC50, in MT-4 cells), X4 HIV-1 (RF):7.4 nM (IC50, in MT-4 cells), X4 HIV-1 (NL4.3AMD3100):2822 nM (IC50, in MT-4 cells), HIV-2 (ROD):12.3 nM (IC50, in MT-4 cells), HIV-2 (EHO):12.3 nM (IC50, in MT-4 cells), X4 HIV-1 (IIIB):12.3 nM (IC50, in MT-4 cells)
In vitro	AMD 3465 hexahydrobromide inhibits binding of 12G5 mAb and CXCL12AF647 to CXCR4, with IC50s of 0.75 nM and 18 nM in SupT1 cells.?AMD 3465 (50 nM) totally blocks CXCL12-induced calcium mobilization, with an IC50 of 17 nM, but shows no effect on the intracellular calcium fluxes elicited by the CCR5 ligands RANTES, LD78β and MIP-1β in U87.CD4.CCR5 cells.?AMD 3465 also potently inhibits the replication of X4 HIV strains (IC50: 1-10 nM), but has no effect on CCR5-using (R5) viruses.?AMD3465 is cytotoxic to the X4 HIV-1 strains IIIB, NL4.3, RF and HE with an IC50 ranging from 6 to 12 nM.?The IC50 for suppression of the HIV-2 strains ROD and EHO is 12.3 nM[1].?AMD 3465 inhibits CXCL-12-induced growth in U87 and Daoy cells.?AMD 3465 treatment stimulates the phosphorylation of Erk1/2 in U87 and Daoy cells[2].
In vivo	Administering 2.5 mg/kg/d of AMD 3465 subcutaneously for five weeks significantly inhibits the growth of U87 glioblastoma multiforme (GBM) and Daoy medulloblastoma xenografts.
Synonyms	GENZ-644494 (hexahydrobromide)

Chemical Properties

Molecular Weight	896.07
Formula	C₂₄H₄₄Br₆N₆
Cas No.	185991-07-5
Smiles	Br.Br.Br.Br.Br.Br.C(NCc1ccccn1)c1ccc(CN2CCCNCCNCCCNCC2)cc1
Relative Density.	1.022g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 38 mg/mL (42.41 mM), Sonication is recommended.

DMSO: 250 mg/mL (279 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (2.23 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.1160 mL	5.5799 mL	11.1598 mL	55.7992 mL
5 mM	0.2232 mL	1.1160 mL	2.2320 mL	11.1598 mL
10 mM	0.1116 mL	0.5580 mL	1.1160 mL	5.5799 mL
20 mM	0.0558 mL	0.2790 mL	0.5580 mL	2.7900 mL

DMSO

	1mg	5mg	10mg	50mg
50 mM	0.0223 mL	0.1116 mL	0.2232 mL	1.1160 mL
100 mM	0.0112 mL	0.0558 mL	0.1116 mL	0.5580 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc