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Anti-ST2/IL-1 RL1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01776
Anti-ST2/IL-1 RL1 Polyclonal Antibody is a Rabbit antibody targeting ST2/IL-1 RL1. Anti-ST2/IL-1 RL1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ST2/IL-1 RL1 Polyclonal Antibody
Anti-ST2/IL-1 RL1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01776
Anti-ST2/IL-1 RL1 Polyclonal Antibody is a Rabbit antibody targeting ST2/IL-1 RL1. Anti-ST2/IL-1 RL1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse |
| Ig Type | IgG |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ST2) Polyclonal Antibody, Unconjugated (TMAB-01776) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ST2) Polyclonal Antibody, Unconjugated (TMAB-01776) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Sample: Kidney (Mouse) Lysate at 40 μg Primary: Anti-ST2 (TMAB-01776) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kDa Observed band size: 61 kDa 4. Sample: 293t (Human) Cell Lysate at 30 μg A549 (Human) Cell Lysate at 30 μg A673 (Human) Cell Lysate at 30 μg MKN45 (Human) Cell Lysate at 30 μg Primary: Anti-ST2 (TMAB-01776) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kDa Observed band size: 61 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Isoform C: Cell membrane.Isoform B: Secreted. Cell membrane; Single-pass type I membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | ST2 is a member of a superfamily containing the Interleukin 1 (IL1) receptor and the Toll-like receptors (TLRs). The TLRs are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. ST2 was originally identified as a protein whose production was stimulated by various proliferation-inducing agents such as PDGF and FGF. More recently, it has been shown to negatively regulate IL1 receptor and Toll-like receptor 4 (TLR4) signaling and to maintain endotoxin tolerance. It has been suggested that the inhibition of TLR4 signaling occurs through the association and sequestering of TLR adaptor molecules such as MyD88 and TIRAP. |
| Immunogen | KLH conjugated synthetic peptide: human ST2 (isoform A,B,C) |
| Antigen Species | Human |
| Gene ID | 9173 |
| Uniprot ID |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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