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Anti-EpCAM/TROP1 Antibody (7L889)

😃Good
Catalog No. TMAY-01073
Alias TROP-1, TROP1, TACSTD1, MK-1, MIC18, M4S1, KSA, KS1/4, HNPCC8, ESA, epithelial cell adhesion molecule, EGP40, EGP314, EGP-2, DIAR5

Anti-EpCAM/TROP1 Antibody (7L889) is a Mouse antibody targeting EpCAM/TROP1. Anti-EpCAM/TROP1 Antibody (7L889) can be used in WB.

Anti-EpCAM/TROP1 Antibody (7L889)

Anti-EpCAM/TROP1 Antibody (7L889)

😃Good
Catalog No. TMAY-01073Alias TROP-1, TROP1, TACSTD1, MK-1, MIC18, M4S1, KSA, KS1/4, HNPCC8, ESA, epithelial cell adhesion molecule, EGP40, EGP314, EGP-2, DIAR5
Anti-EpCAM/TROP1 Antibody (7L889) is a Mouse antibody targeting EpCAM/TROP1. Anti-EpCAM/TROP1 Antibody (7L889) can be used in WB.
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100 μL$198 7-10 days
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Product Introduction

Bioactivity
Description
Anti-EpCAM/TROP1 Antibody (7L889) is a Mouse antibody targeting EpCAM/TROP1. Anti-EpCAM/TROP1 Antibody (7L889) can be used in WB.
AliasTROP-1, TROP1, TACSTD1, MK-1, MIC18, M4S1, KSA, KS1/4, HNPCC8, ESA, epithelial cell adhesion molecule, EGP40, EGP314, EGP-2, DIAR5
Ig Type
Monoclonal Mouse IgG1
Clone
7L889
Reactivity
Human
Verified Activity
1. Anti-EPCAM mouse monoclonal antibody at 1:500 dilution.

-Lane A: A431 Whole Cell Lysate.

-Lane B: MCF7 Whole Cell Lysate.

-Lane C: HCT116 Whole Cell Lysate.

-Lane D: COLO205 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:35 kDa.

-Observed band size:40 kDa.

2. EPCAM was immunoprecipitated using:

-Lane A:0.5 mg MCF-7 Whole Cell Lysate

-0.5 µL anti-EPCAM mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-EPCAM mouse monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 40 kDa.

-Observed band size: 40 kDa
Application
WB
Recommended Dose
WB: 1:500-1:1000
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EpCAM / TROP1 (rh EpCAM; TMPY-01300; NP_002345.1; Met 1-Lys 265). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundEpithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type I transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human EpCAM protein (TMPY-01300)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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