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Di-4-ANEPPS

Name: Di-4-ANEPPS
Brand: TargetMol
SKU: T82567
Rating: 4.5 (1 reviews)

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Di-4-ANEPPS is a fast-response, electrochromic membrane potential fluorescent probe based on a naphthylstyrylpyridinium structure. Its response mechanism involves membrane potential changes causing intramolecular charge transfer, leading to a blue shift in the excitation spectrum. It enables real-time detection of millisecond-scale transient membrane potential changes in excitable cells (e.g., neurons and cardiomyocytes) through ratiometric imaging. Di-4-ANEPPS is non-fluorescent before incorporating into lipid membranes; after incorporation, excitation/emission peaks are approximately in the 465-482 nm/635-686 nm range. Besides being widely used in cardiac electrophysiology and drug cardiac toxicity assessment, it is also a target molecule in studies of singlet oxygen-mediated photooxidative damage at membrane interfaces. Note that Di-4-ANEPPS itself may cause non-reversible interference with cardiac conduction parameters (e.g., heart rate, conduction velocity) at high concentrations or under prolonged illumination and can inhibit sodium current.

Cas No. 90134-00-2

Pack Size	Price	USA Stock	Global Stock	Quantity
1 mg	$287	35 days	35 days
5 mg	$999	35 days	35 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Appearance:Solid

Color:White

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COA

Product Introduction

Bioactivity

Description

In vitro

Treatment of isolated rabbit hearts with Di-4-ANEPPS (2 μM; 20 min) alters cardiac electrical activity, resulting in significant slowing of heart rate and ventricular conduction, as well as reduced ischemia detection capacity [1].
In experiments with human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM), Di-4-ANEPPS (6 μM; 1 min) can be used to assess the electrophysiological properties of hiPSC-CM and to evaluate the effects of drugs on their electrophysiology [2].

Chemical Properties

Molecular Weight	480.66
Formula	C₂₈H₃₆N₂O₃S
Cas No.	90134-00-2
Smiles	O=S(=O)([O-])CCC[N+]1=CC=C(C=CC=2C=CC3=CC(=CC=C3C2)N(CCCC)CCCC)C=C1

Storage & Solubility Information

Storage

Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 10 mg/mL (20.8 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0805 mL	10.4024 mL	20.8047 mL	104.0236 mL
5 mM	0.4161 mL	2.0805 mL	4.1609 mL	20.8047 mL
10 mM	0.2080 mL	1.0402 mL	2.0805 mL	10.4024 mL
20 mM	0.1040 mL	0.5201 mL	1.0402 mL	5.2012 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Related Tags: Di-4-ANEPPS chemical structure | Di-4-ANEPPS in vitro | Di-4-ANEPPS formula | Di-4-ANEPPS molecular weight