Shopping Cart
Remove All
Your shopping cart is currently empty
Di-4-ANEPPS
Copy Product Info
🥰Excellent
Synonyms:
Catalog No. T82567 Copy Product Info
🥰Excellent
Di-4-ANEPPS is a fast-response, electrochromic membrane potential fluorescent probe based on a naphthylstyrylpyridinium structure. Its response mechanism involves membrane potential changes causing intramolecular charge transfer, leading to a blue shift in the excitation spectrum. It enables real-time detection of millisecond-scale transient membrane potential changes in excitable cells (e.g., neurons and cardiomyocytes) through ratiometric imaging. Di-4-ANEPPS is non-fluorescent before incorporating into lipid membranes; after incorporation, excitation/emission peaks are approximately in the 465-482 nm/635-686 nm range. Besides being widely used in cardiac electrophysiology and drug cardiac toxicity assessment, it is also a target molecule in studies of singlet oxygen-mediated photooxidative damage at membrane interfaces. Note that Di-4-ANEPPS itself may cause non-reversible interference with cardiac conduction parameters (e.g., heart rate, conduction velocity) at high concentrations or under prolonged illumination and can inhibit sodium current.
Cas No. 90134-00-2
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 1 mg | $287 | - | In Stock | |
| 5 mg | $999 | 35 days | 35 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
Add to Cart
Add to Quotation
For research use only—not for human use. No sales to individuals. Use as intended only.
Batch Information
Select Batch
Appearance:Solid
Color:White
Contact us for more batch information
Resource Download
Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | Di-4-ANEPPS is a fast-response, electrochromic membrane potential fluorescent probe based on a naphthylstyrylpyridinium structure. Its response mechanism involves membrane potential changes causing intramolecular charge transfer, leading to a blue shift in the excitation spectrum. It enables real-time detection of millisecond-scale transient membrane potential changes in excitable cells (e.g., neurons and cardiomyocytes) through ratiometric imaging. Di-4-ANEPPS is non-fluorescent before incorporating into lipid membranes; after incorporation, excitation/emission peaks are approximately in the 465-482 nm/635-686 nm range. Besides being widely used in cardiac electrophysiology and drug cardiac toxicity assessment, it is also a target molecule in studies of singlet oxygen-mediated photooxidative damage at membrane interfaces. Note that Di-4-ANEPPS itself may cause non-reversible interference with cardiac conduction parameters (e.g., heart rate, conduction velocity) at high concentrations or under prolonged illumination and can inhibit sodium current. |
| In vitro | Treatment of isolated rabbit hearts with Di-4-ANEPPS (2 μM; 20 min) alters cardiac electrical activity, resulting in significant slowing of heart rate and ventricular conduction, as well as reduced ischemia detection capacity [1]. In experiments with human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM), Di-4-ANEPPS (6 μM; 1 min) can be used to assess the electrophysiological properties of hiPSC-CM and to evaluate the effects of drugs on their electrophysiology [2]. |
| Molecular Weight | 480.66 |
| Formula | C28H36N2O3S |
| Cas No. | 90134-00-2 |
| Smiles | O=S(=O)([O-])CCC[N+]1=CC=C(C=CC=2C=CC3=CC(=CC=C3C2)N(CCCC)CCCC)C=C1 |
| Storage | Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Preview
Related Tags: Di-4-ANEPPS chemical structure | Di-4-ANEPPS in vitro | Di-4-ANEPPS formula | Di-4-ANEPPS molecular weight
Generate Quote
Catalog No.: Cas No.:
Add
| Size | Quantity | Unit Price | Amount | Operation |
|---|
Generate Quote
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2026 TargetMol Chemicals Inc. All Rights Reserved.