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Anti-CD20 Antibody (9D637)
😃Good
Catalog No. TMAY-01444
Alias S7, MS4A2, MS4A1, membrane-spanning 4-domains, subfamily A, member 1, LEU-16, CVID5, CD20, Bp35, B1
Anti-CD20 Antibody (9D637) is a Mouse antibody targeting CD20. Anti-CD20 Antibody (9D637) can be used in WB,IHC-P,IP.
Anti-CD20 Antibody (9D637)
Anti-CD20 Antibody (9D637)
😃Good
Catalog No. TMAY-01444Alias S7, MS4A2, MS4A1, membrane-spanning 4-domains, subfamily A, member 1, LEU-16, CVID5, CD20, Bp35, B1
Anti-CD20 Antibody (9D637) is a Mouse antibody targeting CD20. Anti-CD20 Antibody (9D637) can be used in WB,IHC-P,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Chemical Properties
| Description | Anti-CD20 Antibody (9D637) is a Mouse antibody targeting CD20. Anti-CD20 Antibody (9D637) can be used in WB,IHC-P,IP. |
| Alias | S7, MS4A2, MS4A1, membrane-spanning 4-domains, subfamily A, member 1, LEU-16, CVID5, CD20, Bp35, B1 |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 9D637 |
| Reactivity | Human |
| Verified Activity | 1. Anti-CD20 mouse monoclonal antibody at 1:1000 dilution. -Lane A: Raji Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:33 kDa. -Observed band size:36 kDa. 2. Immunochemical staining of human CD20 in human tonsil with mouse monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections. 3. Immunochemical staining of human CD20 in human lymphoma with mouse monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections. 4. Anti-CD20 mouse monoclonal antibody at 1:500 dilution. -Lane A: Raji Whole Cell Lysate. -Lane B: CD20 konckout Raji Whole Cell lysate. -Lysates/proteins at 10 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:33 kDa. -Observed band size:36 kDa. 5. CD20 was immunoprecipitated using: -Lane A:0.5 mg Raji Whole Cell Lysate. -4 µL anti-CD20 mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-CD20 mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 33 kDa. -Observed band size:37 kDa |
| Application | WB,IHC-P,IP |
| Recommended Dose | WB: 1:500-1:2000; IHC-P: 1:100-1:1000; IP: 1-5 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD20 / MS4A1 (rh CD20 / MS4A1; TMPY-02819; NP_068769.2; Glu213-Pro297). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | CD20 (membrane-spanning 4-domains, subfamily A, member 1), also known as MS4A1, is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. CD20 / MS4A1 is expressed in all stages of B cell development except the first and last. CD20 / MS4A1 is present from pre-pre B cells through memory cells, but not on either pro-B cells or plasma cells. It is a B-lymphocyte surface molecule that plays a role in the development and differentiation of B-cells into plasma cells. CD20 / MS4A1may be involved in the regulation of B-cell activation and proliferation. Defects in CD20 / MS4A1 are the cause of immunodeficiency common variable type 5(CVID5). CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections, and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells are usually in the normal range but can be very low.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human CD20 / MS4A1 Protein (TMPY-02819) |
| Antigen Species | Human |
| Biology Area | Cancer Drug Targets |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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