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Arachidonic acid

Name: Arachidonic acid
Brand: TargetMol
SKU: T4129
Price: 39 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T4129Cas No. 506-32-1

Alias Vevodar, Immunocytophyte, Immunocytophyt, arachidonate

Arachidonic acid (Immunocytophyt) is a polyunsaturated omega-6 essential fatty acid that serves as a major component of biological membranes and animal phospholipids. It is synthesized from dietary linoleic acid and acts as a precursor to lipid mediators such as prostaglandins, thromboxanes, and leukotrienes. Arachidonic acid can improve cognitive responses and cardiovascular function and is commonly used to induce paw edema models.

Arachidonic acid

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Purity: 99.78%

Catalog No. T4129Alias Vevodar, Immunocytophyte, Immunocytophyt, arachidonateCas No. 506-32-1

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$39	In Stock	In Stock
10 mg	$55	In Stock	In Stock
25 mg	$77	In Stock	In Stock
50 mg	$97	In Stock	In Stock
100 mg	$122	In Stock	In Stock
200 mg	$178	-	In Stock
500 mg	$297	-	In Stock
1 mL x 10 mM (in DMSO)	$43	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.78%

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Product Introduction

Arachidonic acid AI Summary

Arachidonic acid exhibits a broad range of bioactivities across various biological targets and assays. It shows a strong binding affinity to the adipocyte lipid binding protein with a Kd value of 180 nM and inhibits LTC4 production in THP-1 cells with an IC50 of 4.7 nM. While it demonstrates no significant inhibition on rat glial cell gap junctions and certain bioactivities in whole blood samples, it moderates nicotinic acetylcholine receptor responses in Torpedo, enhancing the original amplitude from 78.0% to 139.0% over 10 minutes. The compound significantly inhibits PAF-induced aggregation of rabbit platelets with an IC50 of approximately 60000.0 nM and shows antiviral activity against HCV with EC50 values of 22100 nM in OR6 cells and much lower IC50 values in Huh7-K2040 cells when tested with delipidated fetal calf serum. Against COX enzymes, Arachidonic acid has Km values of 1000 nM for COX2, 8800 nM for COX1 in bovine seminal microsomes, and 9700 nM for COX2 in sheep placental cotyledons. It also inhibits mPGES1 at 10 µM concentration, showing an IC50 of 300 nM, and demonstrates moderate inhibitory activity against aromatase in different assays. For soybean lipoxygenase 1, Arachidonic acid shows a Km value of 15000 nM and varied levels of activity under different temperatures. It binds to cholera toxin with a high Kd value of 180,000 nM. As an inhibitor of various proteins, it shows bioactivity against FAAH, human tissue factor/human factor 7a, PPAR receptors (PPARalpha, PPARgamma, PPARdelta), and others, while it does not significantly inhibit human CYP51 or other enzymes like pig pancreatic trypsin. It also exhibits inhibitory activity against Staphylococcus aureus FabI and shows weak inhibition of rat TRPV2 channels. Furthermore, Arachidonic acid demonstrates minor antiviral activities against multiple viruses and was identified as an antagonist for Cytochrome P450 3A4. The compound significantly affects enzymes and cellular targets and demonstrates substrate activity towards COX-2. Its varied bioactivities suggest potential applications in anti-inflammatory, antiviral, and other therapeutic areas..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Arachidonic acid (Immunocytophyt) is a polyunsaturated omega-6 essential fatty acid that serves as a major component of biological membranes and animal phospholipids. It is synthesized from dietary linoleic acid and acts as a precursor to lipid mediators such as prostaglandins, thromboxanes, and leukotrienes. Arachidonic acid can improve cognitive responses and cardiovascular function and is commonly used to induce paw edema models.
Targets&IC50	Mg2+-ATPase:90 μM, 3H-ouabain:32 μM, NaK-ATPase:75 μM, L-type Ca2+ channel (cardiac myocytes):8.5 μM
In vitro	METHODS: RAW264.7 and PBMC-derived macrophages were treated with Arachidonic acid (40-80 µM) for 12-24 h and cell viability was determined by CCK8 assay. RESULTS: Arachidonic acid significantly inhibited macrophage viability in a dose-dependent manner within 12 h, and more inhibition was observed between 12 h and 24 h. Arachidonic acid was also shown to inhibit macrophage viability in a dose-dependent manner. [1] METHODS: Human breast cancer cells, MDA-MB-231, were treated with Arachidonic acid (8 µM) for 48 h. Caspase activity was measured using a spectrofluorophotometer. RESULTS: MDA-MB-231 cells stimulated with Arachidonic acid for 48 h showed a significant increase in caspase-3 activity, and Arachidonic acid also induced significant activation of caspase-8 and caspase-9. [2]
In vivo	METHODS: To test the effect on the inflammatory response, Arachidonic acid (150 mg/kg in 1% CMC Na) was administered orally to C57BL/6 mice with high-fat diet (HFD)-induced cardiac injury every two days for eight weeks. RESULTS: Arachidonic acid treatment prevented MD2/TLR4 dimerization, induction of inflammatory factors, and tissue damage through TLR4-mediated inflammatory responses in a high-fat diet obese mouse model. [3]
Synonyms	Vevodar, Immunocytophyte, Immunocytophyt, arachidonate

Chemical Properties

Molecular Weight	304.47
Formula	C₂₀H₃₂O₂
Cas No.	506-32-1
Smiles	CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O
Relative Density.	0.922 g/cm3 at 25℃ (lit.)

Storage & Solubility Information

Storage

keep away from direct sunlight,store under nitrogen,keep away from moisture,store at low temperature | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 262.5 mg/mL (862.15 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 10 mg/mL (32.84 mM), Suspension.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.2844 mL	16.4220 mL	32.8440 mL	164.2198 mL
5 mM	0.6569 mL	3.2844 mL	6.5688 mL	32.8440 mL
10 mM	0.3284 mL	1.6422 mL	3.2844 mL	16.4220 mL
20 mM	0.1642 mL	0.8211 mL	1.6422 mL	8.2110 mL
50 mM	0.0657 mL	0.3284 mL	0.6569 mL	3.2844 mL
100 mM	0.0328 mL	0.1642 mL	0.3284 mL	1.6422 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc