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IGFBP-4 Protein, Mouse, Recombinant (His)

(Synonyms: insulin-like growth factor binding protein 4, IGFBP-4, Deb2, AI875747) Copy Product Info

Synonyms: insulin-like growth factor binding protein 4, IGFBP-4, Deb2, AI875747

Catalog No. TMPY-00837 Copy Product Info
IGFBP-4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 27.2 kDa and the accession number is P47879.
IGFBP-4 Protein, Mouse, Recombinant (His)
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$80-In Stock
10 μg$128-In Stock
20 μg$209-In Stock
50 μg$416-In Stock
100 μg$700-In Stock
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Purity:> 95 % as determined by SDS-PAGE
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Measured in a serum free cell proliferation assay using MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. 1988, Cancer Research 48: 4083.) . The ED50 for this effect is typically 0.1-0.6 μg/ml .
Description
IGFBP-4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 27.2 kDa and the accession number is P47879.
Species
Mouse
Expression System
HEK293 Cells
TagC-His
Accession NumberP47879
ConstructionA DNA sequence encoding the extracellular domain (Met 1-Glu 254) of mouse IGFBP4 (NP_034647.1) precursor was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Asp 22
Protein Purity
> 95 % as determined by SDS-PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with 4 mM HCl to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Synonymsinsulin-like growth factor binding protein 4, IGFBP-4, Deb2, AI875747
Research Background
Chemical Properties
Molecular Weight27.2 kDa (predicted); 38 kDa (reducing condition, due to glycosylation)
Storage & Solubility Information
StorageStore at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: IGFBP-4 Protein, Mouse, Recombinant (His) chemical structure | IGFBP-4 Protein, Mouse, Recombinant (His) molecular weight