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Synonyms: insulin-like growth factor binding protein 4, IGFBP-4, Deb2, AI875747

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $80 | - | In Stock | |
| 10 μg | $128 | - | In Stock | |
| 20 μg | $209 | - | In Stock | |
| 50 μg | $416 | - | In Stock | |
| 100 μg | $700 | - | In Stock |
| Bioactivity | Measured in a serum free cell proliferation assay using MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. 1988, Cancer Research 48: 4083.)
. The ED50 for this effect is typically 0.1-0.6 μg/ml
. |
| Description | IGFBP-4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 27.2 kDa and the accession number is P47879. |
| Species | Mouse |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | P47879 |
| Construction | A DNA sequence encoding the extracellular domain (Met 1-Glu 254) of mouse IGFBP4 (NP_034647.1) precursor was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Asp 22 |
| Protein Purity | > 95 % as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with 4 mM HCl to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | insulin-like growth factor binding protein 4, IGFBP-4, Deb2, AI875747 |
| Research Background |
| Molecular Weight | 27.2 kDa (predicted); 38 kDa (reducing condition, due to glycosylation) |
| Storage | Store at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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