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Anti-CD68 Antibody-PE (9Z25)
🥰Excellent
Catalog No. TMAY-01736P
Anti-CD68 Antibody-PE (9Z25) is a PE-conjugated Mouse antibody targeting CD68. Anti-CD68 Antibody-PE (9Z25) can be used in FCM.
Anti-CD68 Antibody-PE (9Z25)
Anti-CD68 Antibody-PE (9Z25)
🥰Excellent
Catalog No. TMAY-01736P
Anti-CD68 Antibody-PE (9Z25) is a PE-conjugated Mouse antibody targeting CD68. Anti-CD68 Antibody-PE (9Z25) can be used in FCM.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-CD68 Antibody-PE (9Z25) is a PE-conjugated Mouse antibody targeting CD68. Anti-CD68 Antibody-PE (9Z25) can be used in FCM. |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 9Z25 |
| Reactivity | Human |
| Verified Activity | Flow cytometric analysis of human CD68 expression on human whole blood monocytes.The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with PE-conjugated anti-Human CD68. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes. |
| Application | FCM |
| Recommended Dose | 10 μl/Test, 0.1 mg/ml |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD68 (rh CD68; TMPY-02972; P34810-1; Met1-Ser319). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography, and conjugated with PE under optimum conditions, the unreacted PE was removed. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | PBS solution containing 0.5% BSA and 0.03%ProClin300 |
| Research Background | Macrosialin, also known as CD68 and Gp11, is a single-pass type I membrane protein which belongs to theLAMP family. CD68 is highly expressed by blood monocytes and tissue macrophages. It is also expressed in lymphocytes, fibroblasts and endothelial cells. CD68 is expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. CD68 plays a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It is a commonly used marker for macrophages. However, a number of studies have shown that CD68 antibodies react with other hematopoietic and non-hematopoietic cell types, suggesting that CD68 may not be a macrophage-specific antigen. CD68 binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. |
| Conjucates | PE |
| Others Formats | Unconjugated/FITC |
| Antibody Types Available | 3 |
| Immunogen | Recombinant Human CD68 Protein (TMPY-02972) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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