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Anti-Integrin alpha 3 beta 1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00975
Anti-Integrin alpha 3 beta 1 Polyclonal Antibody is a Rabbit antibody targeting Integrin alpha 3 beta 1. Anti-Integrin alpha 3 beta 1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-Integrin alpha 3 beta 1 Polyclonal Antibody
Anti-Integrin alpha 3 beta 1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00975
Anti-Integrin alpha 3 beta 1 Polyclonal Antibody is a Rabbit antibody targeting Integrin alpha 3 beta 1. Anti-Integrin alpha 3 beta 1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF,FCM Reactivity: Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Sheep) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Sheep) |
| Verified Activity | 1. Sample: Cerebrum (Mouse) Lysate at 30 μg Primary: Anti-Integrin Alpha 3 + Beta 1 (TMAB-00975) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kDa Observed band size: 117 kDa 2. Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Integrin Alpha 3 + Beta 1 Polyclonal Antibody, Unconjugated (TMAB-00975) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: Lung (Mouse) Lysate at 30 μg Primary: Anti-Integrin Alpha 3 + Beta 1 (TMAB-00975) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kDa Observed band size: 130 kDa 4. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Integrin Alpha 3 + Beta 1) Polyclonal Antibody, Unconjugated (TMAB-00975) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Blank control: A431. Primary Antibody (green line): Rabbit Anti-Integrin Alpha 3 + Beta 1 antibody (TMAB-00975) Dilution: 1 μg/Test; Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5 μg/Test. Isotype control (orange line): Normal Rabbit IgG Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
| Application | WB,IHC-P,IHC-Fr,IF,FCM |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene belongs to the family of integrins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain, and function as cell surface adhesion molecules. This gene encodes alpha 3 subunit, which undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 subunit to form an integrin that interacts with many extracellular-matrix proteins. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008] |
| Immunogen | KLH conjugated synthetic peptide: human Integrin Alpha 3(976-1025aa) + Beta 1(35-47aa) |
| Antigen Species | Human |
| Gene ID | 3675 |
| Uniprot ID | |
| Biology Area | Alpha |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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