Anti-CXCR4 Polyclonal Antibody is a Rabbit antibody targeting CXCR4. Anti-CXCR4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat

1. Sample:
Lane 1: Blood cell (Mouse) Lysate at 40 μg
Lane 2: LympHnode (Mouse) Lysate at 40 μg
Lane 3: Bone (Rat) Lysate at 40 μg
Lane 4: Spleen (Rat) Lysate at 40 μg
Primary: Anti-CXCR4 at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 44 kDa
2. Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00509) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
3. Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00509) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
4. Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00509) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
5. Paraformaldehyde-fixed, paraffin embedded Human prostate tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00509) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
6. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00509) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
7. Paraformaldehyde-fixed, paraffin embedded Human esophageal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00509) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
8. Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00509) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00509) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: the middle of human CXCR4

Human

Response to hypoxia,Regulatory T Cells,HIV-related,Alpha Chemokine Rec. (CXCR),Hypoxia,Host Virus Interaction,SARS Coronavirus,Axonal Guidance Proteins,Endothelial Markers,Surface Molecules,Surface Molecules