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Anti-CXCR4 Polyclonal Antibody

😃Good
Catalog No. TMAB-00509

Anti-CXCR4 Polyclonal Antibody is a Rabbit antibody targeting CXCR4. Anti-CXCR4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-CXCR4 Polyclonal Antibody

Anti-CXCR4 Polyclonal Antibody

😃Good
Catalog No. TMAB-00509
Anti-CXCR4 Polyclonal Antibody is a Rabbit antibody targeting CXCR4. Anti-CXCR4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$373 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Anti-CXCR4 Polyclonal Antibody is a Rabbit antibody targeting CXCR4. Anti-CXCR4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Sample:
Lane 1: Blood cell (Mouse) Lysate at 40 μg
Lane 2: LympHnode (Mouse) Lysate at 40 μg
Lane 3: Bone (Rat) Lysate at 40 μg
Lane 4: Spleen (Rat) Lysate at 40 μg
Primary: Anti-CXCR4 at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 44 kDa
2. Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00509) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
3. Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00509) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
4. Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00509) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
5. Paraformaldehyde-fixed, paraffin embedded Human prostate tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00509) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
6. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00509) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
7. Paraformaldehyde-fixed, paraffin embedded Human esophageal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00509) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus.
8. Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00509) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CXCR4 Polyclonal Antibody, Unconjugated (TMAB-00509) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Multi-pass membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Monomer. Can form dimers.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: the middle of human CXCR4
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Response to hypoxia,Regulatory T Cells,HIV-related,Alpha Chemokine Rec. (CXCR),Hypoxia,Host Virus Interaction,SARS Coronavirus,Axonal Guidance Proteins,Endothelial Markers,Surface Molecules,Surface Molecules
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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