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Anti-CD68 Polyclonal Antibody 3

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Catalog No. TMAB-00383

Anti-CD68 Polyclonal Antibody 3 is a Rabbit antibody targeting CD68. Anti-CD68 Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-CD68 Polyclonal Antibody 3

Anti-CD68 Polyclonal Antibody 3

😃Good
Catalog No. TMAB-00383
Anti-CD68 Polyclonal Antibody 3 is a Rabbit antibody targeting CD68. Anti-CD68 Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$374 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF,FCM

Reactivity: Mouse,Rat (predicted:Pig)
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Pig)
Verified Activity
1. OCT embedded (Rat spleen), acetone-fixed; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (TMAB-00383) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes, and DAPI for nucleus staining.
2. Blank control: RSC96 cells (blue). Primary Antibody: Rabbit Anti-CD68 antibody (TMAB-00383), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange),used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
3. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-CD68 (TMAB-00383) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 110 kDa
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationIsoform Short: Cell membrane; Single-pass type I membrane protein. Isoform Long: Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
FormulationPreservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
Research BackgroundThis gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: mouse CD68
Antigen Species
Mouse
Gene ID
12514
Uniprot ID
Biology Area
Myeloid Cells,SARS Coronavirus
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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