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Punicalagin

Name: Punicalagin
Brand: TargetMol
SKU: T3921
Price: 38 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T3921Cas No. 65995-63-3

Punicalagin is a major ellagitannin found in pomegranates that is reported to produce antioxidant, anti-inflammatory, and anticancer effects. It has been shown to prevent high-fat diet-induced obesity-associated accumulation of cardiac triglyceride and cholesterol, as well as myocardial damage, via AMPK-mediated modulation of mitochondria and phase II enzymes.

Punicalagin

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Purity: 99.7%

Catalog No. T3921Cas No. 65995-63-3

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$38	In Stock	In Stock
10 mg	$55	In Stock	In Stock
25 mg	$86	In Stock	In Stock
50 mg	$117	In Stock	In Stock
100 mg	$173	In Stock	In Stock
500 mg	$432	-	In Stock
1 mL x 10 mM (in DMSO)	$83	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.7%

Color:Yellow

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COA HPLC HNMR

Product Introduction

Bioactivity

Description	Punicalagin is a major ellagitannin found in pomegranates that is reported to produce antioxidant, anti-inflammatory, and anticancer effects. It has been shown to prevent high-fat diet-induced obesity-associated accumulation of cardiac triglyceride and cholesterol, as well as myocardial damage, via AMPK-mediated modulation of mitochondria and phase II enzymes.
Targets&IC50	PLpro:> 50 μM, 3CLpro:6.192 μg/mL, LoVo cells:100-200 μM, HT29 cells:100-200 μM, SARS-CoV-2:7.20 μM (EC50)
In vitro	METHODS: Human cervical cells ME-180 were treated with Punicalagin (10-100 µM) for 24 h. Cell viability was measured by MTT assay. RESULTS: Punicalagin showed significant concentration-dependent cytotoxicity against ME-180 cells. The cell viability of Punicalagin-treated cells was reduced by approximately 80% compared to control cells. [1] METHODS: Human cervical cancer cells, the HeLa, were treated with Punicalagin (25-100 µM) for 36 h. The cell cycle was detected by Flow cytometry. RESULTS: The number of G1-phase cells increased significantly after 36 h of Punicalagin treatment. [2]
In vivo	METHODS: To study the effects on MTX-induced hepatotoxicity in mice, Punicalagin (25-50 mg/kg, 0.5% CMC) was administered orally to Swiss albino mice once daily for ten days, followed by a single dose of MTX (20 mg/kg) injected intraperitoneally on the seventh day. RESULTS: Punicalagin significantly attenuated MTX-induced elevation of serum aminotransferases, ALP, and LDH, as well as hepatic oxidative stress measures, and enhanced hepatic antioxidant defense. In MTX-induced mouse liver, Punicalagin inhibited oxidative damage, inflammation and apoptosis and upregulated Nrf2. [3]

Chemical Properties

Molecular Weight	1084.72
Formula	C₄₈H₂₈O₃₀
Cas No.	65995-63-3
Smiles	O=C1C=2C=3C=4C5=C(C=6C(=CC(O)=C(O)C6O)C(=O)OCC(O)C(OC(=O)C=7C(C2C(O)=C(O)C3OC5=O)=C(O)C(O)=C(O)C7)C8C(C=O)OC(=O)C=9C(C=%10C(C(=O)O8)=CC(O)=C(O)C%10O)=C(O)C(O)=C(O)C9)C(O)=C(O)C4O1
Relative Density.	2.076 g/cm3 (Predicted)

Storage & Solubility Information

Storage

keep away from direct sunlight | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 100 mg/mL (92.19 mM), Sonication is recommended.

DMSO: 260 mg/mL (239.69 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (4.61 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	0.9219 mL	4.6095 mL	9.2190 mL	46.0948 mL
5 mM	0.1844 mL	0.9219 mL	1.8438 mL	9.2190 mL
10 mM	0.0922 mL	0.4609 mL	0.9219 mL	4.6095 mL
20 mM	0.0461 mL	0.2305 mL	0.4609 mL	2.3047 mL
50 mM	0.0184 mL	0.0922 mL	0.1844 mL	0.9219 mL

DMSO

	1mg	5mg	10mg	50mg
100 mM	0.0092 mL	0.0461 mL	0.0922 mL	0.4609 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc