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Anti-HNF4A Polyclonal Antibody

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Catalog No. TMAB-00877

Anti-HNF4A Polyclonal Antibody is a Rabbit antibody targeting HNF4A. Anti-HNF4A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-HNF4A Polyclonal Antibody

Anti-HNF4A Polyclonal Antibody

😃Good
Catalog No. TMAB-00877
Anti-HNF4A Polyclonal Antibody is a Rabbit antibody targeting HNF4A. Anti-HNF4A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3727-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-HNF4A Polyclonal Antibody is a Rabbit antibody targeting HNF4A. Anti-HNF4A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Sample:
BRL-3a (Rat) Cell Lysate at 30 μg
Primary: Anti-HNF4A (TMAB-00877) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
2. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-HNF4A Polyclonal Antibody, Unconjugated (TMAB-00877) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HNF4A) Polyclonal Antibody, Unconjugated (TMAB-00877) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Sample: Hcclm3 Cell (Human) Lysate at 40 μg
Primary: Anti-HNF4A (TMAB-00877) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by the HNF4 gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non insulin dependent diabetes mellitus type I. At least three different transcript variants encoding three different isoforms have been found for this gene.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human HNF4 alpha
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Energy Metabolism,Heart disease,Diabetes,Cancer,Cytochromes,Lipid metabolism,Lipases,Cholesterol Metabolism,Energy Metabolism,Pol II Transcription,Liver development,Cytochromes,Cholesterol Metabolism,Extrinsic,Diabetes associated,Metabolism of lipids and lipoproteins
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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