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Amitriptyline hydrochloride

Name: Amitriptyline hydrochloride
Brand: TargetMol
SKU: T0678
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T0678Cas No. 549-18-8

Alias Tryptizol, Domical, Annoyltin, Amitriptyline HCl

Amitriptyline hydrochloride (Annoyltin) is the hydrochloride salt of the tricyclic dibenzocycloheptadiene amitriptyline with antidepressant and antinociceptive activities.

Amitriptyline hydrochloride

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Purity: 99.94%

Catalog No. T0678Alias Tryptizol, Domical, Annoyltin, Amitriptyline HClCas No. 549-18-8

Amitriptyline hydrochloride (Annoyltin) is the hydrochloride salt of the tricyclic dibenzocycloheptadiene amitriptyline with antidepressant and antinociceptive activities.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
5 g	$30	-	In Stock
1 mL x 10 mM (in DMSO)	$39	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:99.94%

Color:White to Yellow

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COA HNMR HPLC

Product Introduction

Bioactivity

Description	Amitriptyline hydrochloride (Annoyltin) is the hydrochloride salt of the tricyclic dibenzocycloheptadiene amitriptyline with antidepressant and antinociceptive activities.
Targets&IC50	H4 receptor:33.6 nM, H3 receptor:1 μM, 5-HT receptor:3.45 nM, σ1 receptor:287 nM, DAT:2.58 μM (Ki), 5-HT2B receptor:40 nM, 5-HT2C receptor:6 nM, Norepinephrine receptor:13.3 nM, 5-HT4:7.31 nM, H1 Receptor:1.1 nM, 5-HT1A receptor:450 nM, 5-HT2:235 nM, mAChR:7.2 nM, Adrenoceptor:24 nM, 5-HT2A receptor:4 nM, 5-HT1B receptor:40 nM
In vitro	Amitriptyline inhibits Forskolin-stimulated cyclic AMP accumulation with EC50 values of 16.2 μM in intact CHO/DOR cells. Amitriptyline causes a concentration-dependent stimulation of ERK1/2 and GSK-3β phosphorylation with EC50 values of 9.0 μM in CHO/DOR cells. Amitriptyline (15 μM) causes a stimulation of ERK1/2 phosphorylation in C6 cells. Amitriptyline (30 μM) inhibits Forskolin-stimulated adenylyl cyclase activity and antagonizes ( )-U50,488 inhibitory effect in rat nucleus accumbens. [5] Amitriptyline binds the extracellular domain of both TrkA and TrkB and promotes TrkA-TrkB receptor heterodimerization. Amitriptyline (< 500 nM) promotes TrkA autophosphorylation in primary neurons and induces neurite outgrowth in PC12 cells. Amitriptyline selectively protects T17 cells from apoptosis with EC50 of 50 nM. [6]
In vivo	Amitriptyline (15 mg/kg, i.p.) activates TrkA and TrkB receptors and significantly reduces kainic acid-triggered neuronal cell death in mice. [6] Amitriptyline (15 mg/kg and 30 mg/kg, i.p.) dose-dependently decreases the immobility time in the forced swimming test (FST) of mice. Amitriptyline (15 mg/kg, i.p.) shows a significant 24-h rhythm in the immobility time in the forced swimming test (FST) of mice. [7] Amitriptyline (1 mg/kg and 3 mg/kg) significantly increases the total distance travelled of mice in novel cages. Amitriptyline (10 mg/kg p.o., twice daily) considerably attenuates the hypothermic response to 8-OHDPAT and mCPP in mice. Amitriptyline (10 mg/kg p.o., twice daily) significantly reduces serotonin transporter density by approximately 20% in cortex of mice. [8]
Synonyms	Tryptizol, Domical, Annoyltin, Amitriptyline HCl

Chemical Properties

Molecular Weight	313.86
Formula	C₂₀H₂₄ClN
Cas No.	549-18-8
Smiles	Cl.CN(C)CC\C=C1\c2ccccc2CCc2ccccc12
Relative Density.	1.076g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 250 mg/mL (796.53 mM), Sonication is recommended.

H2O: 12 mg/mL (38.23 mM), Sonication is recommended.

Ethanol: 59 mg/mL (187.98 mM), Sonication is recommended.

Solution Preparation Table

H2O/Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.1861 mL	15.9307 mL	31.8613 mL	159.3067 mL
5 mM	0.6372 mL	3.1861 mL	6.3723 mL	31.8613 mL
10 mM	0.3186 mL	1.5931 mL	3.1861 mL	15.9307 mL
20 mM	0.1593 mL	0.7965 mL	1.5931 mL	7.9653 mL

Ethanol/DMSO

	1mg	5mg	10mg	50mg
50 mM	0.0637 mL	0.3186 mL	0.6372 mL	3.1861 mL
100 mM	0.0319 mL	0.1593 mL	0.3186 mL	1.5931 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc