B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 μg | 5 days | $ 418.00 | |
500 μg | 5 days | $ 1,670.00 | |
1 mg | 5 days | $ 2,800.00 |
Description | B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively. |
Species | Cynomolgus |
Expression System | HEK293 |
Tag | C-His |
Accession Number | G7NXR4 |
Synonyms | B72, MGC34413, LAB72, B7-2, FUN-1, B7-2 antigen, CD86, BU63, CD28LG2, B70, CD86 molecule |
Construction | Recombinant Cynomolgus B7-2/CD86 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Tyr26-His240.[Accession |G7NXR4] |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Molecular Weight | The protein has a predicted MW of 26.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
Endotoxin | Less than 1EU per μg by the LAL method. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability & Storage |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively. |
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Please read the User Guide of Recombinant Proteins for more specific information.
B7-2 Protein, Cynomolgus, Recombinant (His) B72 MGC34413 MGC 34413 CD 86 B 70 LAB72 B-72 CD-86 B7-2 FUN-1 B7-2 antigen CD86 B 72 LAB 72 BU63 CD28LG2 LAB-72 B70 FUN 1 BU-63 MGC-34413 CD86 molecule BU 63 B-70 FUN1 recombinant recombinant-proteins proteins protein