Antibody Type: Mouse Monoclonal

Application: WB, ICC/IF, IP

Reactivity: Human

Mouse IgG1

5R992

Human

1. SUB1 was immunoprecipitated using:
-Lane A:0.5 mg HepG2 Whole Cell Lysate.
-2 µL anti-SUB1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-SUB1 mouse monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 17 kDa.
-Observed band size: 17 kDa.
2. Anti-SUB1 mouse monoclonal antibody at 1:500 dilution.
-Lane A: NIH-3T3 Whole Cell Lysate.
-Lane B: Raji Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:14 kDa.
-Observed band size:16 kDa.
3. Immunofluorescence staining of Human SUB1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human SUB1 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody(red) and counterstained with DAPI(blue). Positive staining was localized to nucleus.

WB,ICC/IF,IP

WB: 1:500-1:2000; ICC-IF: 1:20-1:100; IP: 1-4 μL/mg of lysate

Monoclonal

Unconjugated

Recombinant Protein: Human SUB1 / PC4 protein (TMPY-03395)

Human