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Anti-PC4/SUB1 Antibody (5R992)

😃Good
Catalog No. TMAY-02719
Alias SUB1 homolog, transcriptional regulator, SUB1, RPO2TC1, PC4, P15, p14

Anti-PC4/SUB1 Antibody (5R992) is a Mouse antibody targeting PC4/SUB1. Anti-PC4/SUB1 Antibody (5R992) can be used in WB,ICC/IF,IP.

Anti-PC4/SUB1 Antibody (5R992)

Anti-PC4/SUB1 Antibody (5R992)

😃Good
Catalog No. TMAY-02719Alias SUB1 homolog, transcriptional regulator, SUB1, RPO2TC1, PC4, P15, p14
Anti-PC4/SUB1 Antibody (5R992) is a Mouse antibody targeting PC4/SUB1. Anti-PC4/SUB1 Antibody (5R992) can be used in WB,ICC/IF,IP.
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100 μL188 € 7-10 days
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Product Introduction

Bioactivity
Description
Anti-PC4/SUB1 Antibody (5R992) is a Mouse antibody targeting PC4/SUB1. Anti-PC4/SUB1 Antibody (5R992) can be used in WB,ICC/IF,IP.
AliasSUB1 homolog, transcriptional regulator, SUB1, RPO2TC1, PC4, P15, p14
Ig Type
Monoclonal Mouse IgG1
Clone
5R992
Reactivity
Human
Verified Activity
1. SUB1 was immunoprecipitated using:

-Lane A:0.5 mg HepG2 Whole Cell Lysate.

-2 µL anti-SUB1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-SUB1 mouse monoclonal antibody, at 1:100 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 17 kDa.

-Observed band size: 17 kDa.

2. Anti-SUB1 mouse monoclonal antibody at 1:500 dilution.

-Lane A: NIH-3T3 Whole Cell Lysate.

-Lane B: Raji Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:14 kDa.

-Observed band size:16 kDa.

3. Immunofluorescence staining of Human SUB1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human SUB1 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody(red) and counterstained with DAPI(blue). Positive staining was localized to nucleus.
Application
WB,ICC/IF,IP
Recommended Dose
WB: 1:500-1:2000; ICC-IF: 1:20-1:100; IP: 1-4 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SUB1 / PC4 (rh SUB1 / PC4; TMPY-03395; P53999; Met1-Leu127). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundSUB1 belongs to the transcriptional coactivator PC4 family. It is a general coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. SUB1 binds single-stranded DNA. Single-stranded DNA-binding proteins play many roles in nucleic acid metabolism, but their importance during transcription remains unclear. SUB1 exhibits strong genetic interactions with factors necessary for promoter melting. It localizes near the transcription bubble in vitro and binds to promoters in vivo dependent upon preinitiation complexes assembly. SUB1 interacts with the nontemplate strand of RNApII complexes during initiation. It may also be involved in stabilizing the multiprotein transcription complex.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human SUB1 / PC4 protein (TMPY-03395)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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