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Anti-CD20 Antibody (1R324)
😃Good
Catalog No. TMAB-00343
Anti-CD20 Antibody (1R324) is a Rabbit antibody targeting CD20. Anti-CD20 Antibody (1R324) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CD20 Antibody (1R324)
Anti-CD20 Antibody (1R324)
😃Good
Catalog No. TMAB-00343
Anti-CD20 Antibody (1R324) is a Rabbit antibody targeting CD20. Anti-CD20 Antibody (1R324) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μL | $155 | 7-10 days | |
| 50 μL | $271 | 7-10 days | |
| 100 μL | $489 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat |
| Ig Type | IgG |
| Clone | 1R324 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: Lane 1: Human Raji cell lysates Primary: Anti-CD20 (TMAB-00343) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kDa Observed band size: 35 kDa 2. Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD20) Monoclonal Antibody, Unconjugated (TMAB-00343) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD20) Monoclonal Antibody, Unconjugated (TMAB-00343) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD20) Monoclonal Antibody, Unconjugated (TMAB-00343) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD20) Monoclonal Antibody, Unconjugated (TMAB-00343) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD20) Monoclonal Antibody, Unconjugated (TMAB-00343) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD20) Monoclonal Antibody, Unconjugated (TMAB-00343) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CD20) Monoclonal Antibody, Unconjugated (TMAB-00343) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded (Diffuse large B-cell lymphoma); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (phospho-GSK-3 Beta (Ser9)) Monoclonal Antibody, Unconjugated (TMAB-00343) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:1000-5000; IHC-P: 1:200-1000; IHC-Fr: 1:400-800; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Multi-pass membrane protein. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | CD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit. |
| Immunogen | KLH conjugated synthetic peptide: human CD20 |
| Antigen Species | Human |
| Gene ID | 931 |
| Uniprot ID | |
| Biology Area | Tumor-associated antigens,CD,Human Lineage Negative |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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