Shopping Cart

Your shopping cart is currently empty

Formononetin

Name: Formononetin
Brand: TargetMol
SKU: T0724
Price: 45 USD
Availability: InStock
Rating: 5 (10 reviews)

Copy Product Info

🥰Excellent

Catalog No. T0724Cas No. 485-72-3

Alias Formononetol, Flavosil, Biochanin B

Formononetin (Flavosil) is an O-methylated isoflavone and a phytoestrogen from the root of Astragalus membranaceus.

Formononetin

Copy Product Info

🥰Excellent

Purity: 99.94%

Catalog No. T0724Alias Formononetol, Flavosil, Biochanin BCas No. 485-72-3

Formononetin (Flavosil) is an O-methylated isoflavone and a phytoestrogen from the root of Astragalus membranaceus.

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$45	In Stock	In Stock
50 mg	$56	In Stock	In Stock
100 mg	$77	In Stock	In Stock
500 mg	$174	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$50	In Stock	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:99.94%

Color:White

Resource Download

COA HNMR LCMS

Product Introduction

Formononetin AI Summary

Formononetin demonstrates various bioactivities, including strong antiproliferative effects against multiple human cancer cell lines such as NCI60 (GI50: 50,000 nM), HCT116 (GI50: 64,000 nM), HT29 (GI50: 74,000 nM), MDA-MB-468 (GI50: 27,200 nM), and MCF7 (GI50: 81,400 nM). It also shows inhibitory action against CYP1A1 (IC50 < 100,000 nM) and exhibits significant SGLT2 inhibition in COS1 cells (75.4% inhibition). The compound reveals antimicrobial properties, effectively inhibiting Escherichia coli (MIC: 100 µg/mL), Penicillium expansum (MIC: 50 µg/mL), and Aspergillus niger (MIC: 100 µg/mL), though it is less effective against some other bacterial and fungal species. Formononetin also exhibits cytotoxicity in U937 cells, with IC50 values of 50,000 nM at 24 hours, 44,000 nM at 48 hours, and 45,000 nM at 72 hours, and against PANC1 cells under nutrient-deprived conditions (CD100 > 100 µM). It displays neuropharmacological potential by displacing [3H]diazepam from benzodiazepine receptors at an IC50 of 700,000 nM, suggesting potential anxiolytic or sedative effects. Additionally, the compound exhibits notable antineoplastic activity, demonstrating potent cytotoxicity against human cancer lines like A549, MCF7, and HT-29 (ED50 values of 2.87, 1.96, and 6.43 µg/mL, respectively), and shows strong antitrypanosomal properties against Trypanosoma brucei brucei (IC50: 90,200 nM). Moreover, it has antiviral activities against SARS-CoV-2, with notable inhibition percentages in VERO-6 cells. The compound also demonstrates anti-inflammatory and antioxidant activities, inhibiting nitric oxide production in mouse RAW264.7 cells (IC50: 56,100 nM) and BV2 cells (weak potency). Its reduction of Fe3+ to Fe2+ (IC50: 62.57 µg/mL) and scavenging of ABTS (IC50: 77.42 µg/mL) and DPPH radicals (IC50: 43.51 µg/mL) further highlight its antioxidant potential. Additionally, it modulates various molecular targets, including human PPARalpha and PPARgamma receptors, enhancing their activities significantly. In enzymatic activities, Formononetin inhibits both electric eel AChE and horse BChE, suggesting cholinesterase inhibition potential, and shows moderate inhibition of mouse MAO-A and MAO-B enzymes. Lastly, it reveals anti-obesity effects in a mouse model, reducing body weight by 18.8% at 50 mg/kg over eight weeks. Overall, Formononetin exhibits a wide array of bioactivities, including antiproliferative, antimicrobial, neuropharmacological, antiviral, anti-inflammatory, antioxidant, and enzyme inhibitory properties, making it a promising candidate for further therapeutic investigations..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Formononetin (Flavosil) is an O-methylated isoflavone and a phytoestrogen from the root of Astragalus membranaceus.
Targets&IC50	FGFR2:4.31 μM (IC50)
Synonyms	Formononetol, Flavosil, Biochanin B

Chemical Properties

Molecular Weight	268.26
Formula	C₁₆H₁₂O₄
Cas No.	485-72-3
Smiles	COC1=CC=C(C=C1)C1=COC2=CC(O)=CC=C2C1=O
Relative Density.	1.329 g/cm3

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 35 mg/mL (130.47 mM), Sonication and heating are recommended.

H2O: < 1 mg/mL (insoluble or slightly soluble)

In Vivo Formulation

10% DMSO+90% Corn Oil: 2 mg/mL (7.46 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.7277 mL	18.6386 mL	37.2773 mL	186.3863 mL
5 mM	0.7455 mL	3.7277 mL	7.4555 mL	37.2773 mL
10 mM	0.3728 mL	1.8639 mL	3.7277 mL	18.6386 mL
20 mM	0.1864 mL	0.9319 mL	1.8639 mL	9.3193 mL
50 mM	0.0746 mL	0.3728 mL	0.7455 mL	3.7277 mL
100 mM	0.0373 mL	0.1864 mL	0.3728 mL	1.8639 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc