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Anti-HSD3B1 Polyclonal Antibody

Catalog No. TMAB-00891 Copy Product Info
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Anti-HSD3B1 Polyclonal Antibody is a Rabbit antibody targeting HSD3B1. Anti-HSD3B1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P.

Anti-HSD3B1 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-00891

Anti-HSD3B1 Polyclonal Antibody is a Rabbit antibody targeting HSD3B1. Anti-HSD3B1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P.

Anti-HSD3B1 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2207-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5297-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-HSD3B1 Polyclonal Antibody is a Rabbit antibody targeting HSD3B1. Anti-HSD3B1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P.
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Dog,Cow,Horse)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (TMAB-00891) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (TMAB-00891) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (TMAB-00891) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (TMAB-00891) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (TMAB-00891) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSD3B1) Polyclonal Antibody, Unconjugated (TMAB-00891) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationEndoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.
Tissue SpecificityPlacenta and skin. Predominantly expressed in mammary gland tissue.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundThe protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human HSD3B1
Antigen Species
Human
Gene Name
HSD3B1
Gene ID
Protein Name
3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase type 1
Uniprot ID
Biology Area
Hormone biosynthesis,Metabolism of lipids and lipoproteins,Hormone biosynthesis,Lipid metabolism,Cancer,Hormones
Chemical Properties
Molecular WeightTheoretical: 41 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Keywords

δ-5-3-ketosteroid isomeraseTrophoblast antigen FDO161GSteroid δ-isomeraseSteroid d-isomeraseSteroid Delta-isomeraseHSDB3AHSD3B1HSD3B 1Dihydrotestosterone oxidoreductaseDelta-5-3-ketosteroid isomerased-5-3-ketosteroid isomerase3-β-hydroxy-δ(5)-steroid dehydrogenase3-β-hydroxysteroid 3-dehydrogenase3-β-hydroxy-Delta(5)-steroid dehydrogenase3-β-hydroxy-d(5)-steroid dehydrogenase3-β-hydroxy-5-ene steroid dehydrogenase3-β-HSD I3-b-hydroxy-δ(5)-steroid dehydrogenase3-b-hydroxysteroid 3-dehydrogenase3-b-hydroxy-Delta(5)-steroid dehydrogenase3-b-hydroxy-d(5)-steroid dehydrogenase3-b-hydroxy-5-ene steroid dehydrogenase3-b-HSD I3BH3-beta-hydroxy-δ(5)-steroid dehydrogenase3-beta-hydroxysteroid 3-dehydrogenase3-beta-hydroxy-Delta(5)-steroid dehydrogenase3-beta-hydroxy-d(5)-steroid dehydrogenase3-beta-hydroxy-5-ene steroid dehydrogenase3-beta-HSD I3 β-hydroxysteroid dehydrogenase/δ 5-->4-isomerase type I (3-β-HSD I)3 β-hydroxysteroid dehydrogenase/δ 5-->4-isomerase type 13 β-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I (3-β-HSD I)3 β-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 13 β-hydroxysteroid dehydrogenase/d 5-->4-isomerase type I (3-β-HSD I)3 β-hydroxysteroid dehydrogenase/d 5-->4-isomerase type 13 β-HSDI3 b-hydroxysteroid dehydrogenase/δ 5-->4-isomerase type I (3-b-HSD I)3 b-hydroxysteroid dehydrogenase/δ 5-->4-isomerase type 13 b-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I (3-b-HSD I)3 b-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 13 b-hydroxysteroid dehydrogenase/d 5-->4-isomerase type I (3-b-HSD I)3 b-hydroxysteroid dehydrogenase/d 5-->4-isomerase type 13 b-HSDI3 beta-hydroxysteroid dehydrogenase/δ 5-->4-isomerase type I (3-beta-HSD I)3 beta-hydroxysteroid dehydrogenase/δ 5-->4-isomerase type 13 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I (3-beta-HSD I)3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 13 beta-hydroxysteroid dehydrogenase/d 5-->4-isomerase type I (3-beta-HSD I)3 beta-hydroxysteroid dehydrogenase/d 5-->4-isomerase type 13 beta-HSDI
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