5-(6)-Carboxyfluorescein

5(6)-Carboxyfluorescein (5-(and-6)-Carboxyfluorescein mixed isomers) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid.

5(6)-Carboxyfluorescein diisobutyrate, a protected form of 5(6)-carboxyfluorescein, serves as a fluorescence pH indicator [1].

CFSE (CFDA-SE) is a fluorescent dye with cell membrane permeability. CFSE irreversibly binds to intracellular proteins in living cells and is used for the detection of cell proliferation. The labeled cells fluoresce in green color with excitation wavelength of 488 nm and emission wavelength of 518 nm.

5(6)-FITC (Fluorescein isothiocyanate), or Fluorescein 5(6)-isothiocyanate, is a versatile amine-reactive derivative of fluorescein dye. It serves as an effective label for antibodies and other probes, finding utility in fluorescence microscopy, flow cytometry, and immunofluorescence-based assays such as ELISA and Western blotting.

5(6)-TAMRA SE (5(6)-Carboxytetramethylrhodamine N-succinimidyl ester) is the amine-reactive, mixed isomer form of TAMRA. 5(6)-TAMRA SE is a dye for oligonucleotide labeling and automated DNA sequencing applications.

6-ROX (6-Carboxy-X-rhodamine) is a fluorescent oligonucleotide marker and acts as an acceptor molecule coupled to 5-FAM as the donor in FRET imaging with excitation of 514nm and emission of 525nm.

5(6)-Aminofluorescein (5(6)-AFM) is a high-purity, high-quality chemical utilized as a molecular probe and a significant fluorescent agent.

5(6)-CFDA (5-(6)-Carboxyfluorescein diacetate) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activities, which is required to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product.

5(6)-FAM SE (5(6)-Carboxyfluorescein N-hydroxysuccinimide ester) is an amine-reactive green fluorescent dye. 5(6)-FAM SE is widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.

5(6)-ROX, a nucleic acid fluorescent label, serves as a reference dye in real-time polymerase chain reaction (PCR) applications.

5(6)-TAMRA (5(6)-Carboxytetramethylrhodamine) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. It is bright, orange-fluorescent dye produces conjugates with absorption emission maxima of ~555 580 nm.

BCECF is a pH-sensitive fluorescent dye and a cytosolic pH indicator. It can be used for detecting intracellular pH levels.

5-FAM-Ahx-LL-37 TFA is a 5-FAM labeled version of LL-37, human. The carboxyfluorescein moiety is attached via 6-Aminohexanoic acid (Ahx). LL-37, human, is a 37-residue amphipathic antimicrobial peptide, derived from an antibiotic, possessing broad-spectrum antimicrobial activity.

bis-ANS is a high-affinity non-covalent extrinsic fluorescent dye used to analyze protein conformation.[1] Its predominant interaction with proteins is through its hydrophobic phenyl and naphthyl rings.[2] bis-ANS has an excitation maxima of 390 nm.[3] It has an emission maximum of 523 nm when free in solution but undergoes a blue shift with an increase in fluorescence intensity when bound to protein; for example, when bound to intestinal fatty acid binding protein (FAPB2) it has emission maxima of 484-496 nm. bis-ANS has been used to label mechanically damaged neurons in acute brain slices.4 It also potently inhibits microtubule assembly.[5],[6]

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate is an oxidant-sensitive and cell-permeable fluorescent probe.1Base hydrolysis of the ester bonds by intracellular esterases releases the acetate groups and the resulting compound, 5(6)-carboxy-2',7',-dichlorofluorescein, is oxidized by reactive oxygen species (ROS) and nitric oxide (NO) and displays excitation emission maxima of 504 and 530 nm, respectively, which can be quantified as a measure of oxidant production. 1.Hempel, S.L., Buettner, G.R., O'Malley, Y.Q., et al.Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: Comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123Free Radic. Biol. Med.27(1-2)146-159(1999)

NHS-5(6)Carboxyrhodamine is a fluorescent dye used for precise protein labeling through fluorescence, ensuring accurate dye-to-protein ratios even under native conditions.

HKSOX-1m (5 6-mixture) is a mitochondria-targeting O2 fluorescent probe with excitation emission wavelengths of 509 534 nm (green), offering exceptional selectivity and sensitivity for O2 across a wide pH range and in the presence of strong oxidants and prevalent reductants within cells.

Biotin-11-dUTP [tetra(triethylammonium) salt] [(2S,3S,4R,5R,6R)-5-(2,4-dioxopyrimidin-1-yl)-4-hydroxy-6-(hydroxymethyl)-2-[(3aR,4S,6R,6aS)-2-oxohexahydro-2H-thieno[3,4-d]imidazol-4-yl]-tetrahydro-2H-furo[3,2-d][1,3,2]dioxaphosphorin-3-yl]phosphonate] is used as a fluorescent substitute for dTTP.

Sodium 2,2',2'',2'''-((((1,1-dioxido-3H-benzo[c][1,2]oxathiole-3,3-diyl)bis(6-hydroxy-5-isopropyl-2-methyl-3,1-phenylene))bis(methylene))bis(azanetriyl))tetraacetate is a useful organic compound for research related to life sciences. The catalog number is T65315 and the CAS number is 1945-77-3.

HKSOX-1r (5 6-mixture) is a fluorescent probe specifically designed for the imaging and detection of endogenous superoxide in live cells and in vivo applications, demonstrating superior selectivity and sensitivity to the superoxide anion radical [1]. For storage, it must be protected from light.

Flutax-2 (5 6-mixture), an active fluorescent derivative of paclitaxel, binds to polymerized α,β tubulin dimers, with an excitation emission wavelength of 496 524 nm. Derived from paclitaxel, a diterpenoid secondary metabolite from Taxus species utilized in various cancer research [1], it requires storage protection from light.

HKSOX-1m hydrobromide (5 6-mixture), a mitochondria-targeted O2•− fluorescent probe (Ex Em=509 534nm; green), demonstrates exceptional selectivity and sensitivity for O2•− across a wide pH spectrum, robustly distinguishing it from strong oxidants and prevalent cellular reductants [1].

Mca-(endo-1α-Dap(Dnp))-TNF-α(-5 to +6) amide (human) TFA is a peptide that functions as a fluorescence resonance energy transfer (FRET)-based substrate, with its activity determined by the change in fluorescence intensity following cleavage [1].

5'6-FAM-GGUGAAAGACGAUGGACAATT,UUGUCCAUCGUCUUUCACCTT is a double-stranded ribonucleoside modified with the fluorescent molecule 5'6-FAM, a combination of 5-FAM and 6-FAM, exhibiting excitation emission wavelengths at 490 nm 520 nm [1].