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BCECF

Name: BCECF
Brand: TargetMol
SKU: T18922
Price: 54 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T18922Cas No. 85138-49-4

Alias 2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein

BCECF is a pH-sensitive fluorescent dye and a cytosolic pH indicator. It can be used for detecting intracellular pH levels.

BCECF

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Catalog No. T18922Alias 2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluoresceinCas No. 85138-49-4

BCECF is a pH-sensitive fluorescent dye and a cytosolic pH indicator. It can be used for detecting intracellular pH levels.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 mg	$54	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Appearance:Solid

Color:White to Orange

Resource Download

COA

Product Introduction

Bioactivity

Description	BCECF is a pH-sensitive fluorescent dye and a cytosolic pH indicator. It can be used for detecting intracellular pH levels.
In vitro	Methods: 1. 2-20mM BCECF mother liquor was prepared in anhydrous DMSO. 2. Use Hanks or PBS to prepare 5-50µM BCECF dye. 3. Add the configured BCECF dye to the cell culture plate. 1000 μL/well (6-well plate),100 μL/well (96-well plate) or 25 μL/well (384-well plate). 4, Use a fluorescence microscope or fluorescent plate reader to measure at Ex/Em = 490/535 nm or 430/535 nm. Results: The new use of BCECF as a fluorescent marker for hydrogen bodies (the first such marker) was demonstrated. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein

Chemical Properties

Molecular Weight	520.44
Formula	C₅₄H₄₀O₂₂
Cas No.	85138-49-4
Smiles	O=C(O1)C2=C(C=C(C(O)=O)C=C2)C31C4=C(C=C(O)C(CCC(O)=O)=C4)OC5=CC(O)=C(CCC(O)=O)C=C53 O=C(O1)C2=C(C=CC(C(O)=O)=C2)C31C4=C(C=C(O)C(CCC(O)=O)=C4)OC5=CC(O)=C(CCC(O)=O)C=C53
Relative Density.	1.67g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: < 0.1 mg/mL (insoluble)

DMSO: 50 mg/mL (96.07 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2.5 mg/mL (4.8 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.9215 mL	9.6073 mL	19.2145 mL	96.0726 mL
5 mM	0.3843 mL	1.9215 mL	3.8429 mL	19.2145 mL
10 mM	0.1921 mL	0.9607 mL	1.9215 mL	9.6073 mL
20 mM	0.0961 mL	0.4804 mL	0.9607 mL	4.8036 mL
50 mM	0.0384 mL	0.1921 mL	0.3843 mL	1.9215 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

BCECF2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein