11-Oxomogroside II A1 is an oxidized cucurbitane triterpenoid glycoside derived from luo han guo (Siraitia grosvenorii), typically isolated from its ethanol extract. In vitro, 11-Oxomogroside II A1 inhibits TPA-induced activation of the Epstein–Barr virus early antigen (EBV-EA) and exhibits weak inhibitory effects on nitric oxide donor-induced responses.

Methods: After seeding EBV-positive human Burkitt’s lymphoma Raji cells, TPA (32 μM), 11-Oxomogroside II A1 (at ratios of 1000, 500, 100, and 10 μM relative to 32 μM TPA), n-butyric acid, and incubated for 48 h. The percentage of EBV-EA-positive cells was detected using indirect immunofluorescence, while Raji cell viability was simultaneously assessed via trypan blue staining. The half-maximal inhibitory concentration (IC₅₀) was then calculated.
Results: At a 1000-fold molar concentration, 11-Oxomogroside II A1 induced EBV-EA in only 8.5% of cells (compared to 100% in the TPA-positive control), while Raji cell survival reached 70%, indicating no significant cytotoxicity; at molar ratios of 500×, 100×, and 10×, the EBV-EA induction rates were 25.0%, 80.6%, and 100%, respectively. [1]
Methods: After seeding normal human Chang liver hepatocytes, NOR 1 (350 nm) and 11-Oxomogroside II A1 (350 nm) were added. After 20 minutes of incubation, the amount of NO generated in the system was measured; the inhibition rate of the test substance (I.R.) was calculated using the inhibition rate of NOR 1’s self-activation as the reference value of 1.0.
Results: At the test concentration of 350 nm, the inhibition rate of NOR 1 activation was I.R. = 1.4, indicating NO scavenging/inhibitory activity.[1]