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5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate

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Catalog No. T38123Cas No. 127770-45-0

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate is an oxidant-sensitive and cell-permeable fluorescent probe.1Base hydrolysis of the ester bonds by intracellular esterases releases the acetate groups and the resulting compound, 5(6)-carboxy-2',7',-dichlorofluorescein, is oxidized by reactive oxygen species (ROS) and nitric oxide (NO) and displays excitation/emission maxima of 504 and 530 nm, respectively, which can be quantified as a measure of oxidant production.

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate

😃Good
Catalog No. T38123Cas No. 127770-45-0
5(6)-Carboxy-2',7'-dichlorofluorescein diacetate is an oxidant-sensitive and cell-permeable fluorescent probe.1Base hydrolysis of the ester bonds by intracellular esterases releases the acetate groups and the resulting compound, 5(6)-carboxy-2',7',-dichlorofluorescein, is oxidized by reactive oxygen species (ROS) and nitric oxide (NO) and displays excitation/emission maxima of 504 and 530 nm, respectively, which can be quantified as a measure of oxidant production.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 mg$14335 days35 days
50 mg$22335 days35 days
100 mg$29635 days35 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Product Introduction

Bioactivity
Description
5(6)-Carboxy-2',7'-dichlorofluorescein diacetate is an oxidant-sensitive and cell-permeable fluorescent probe.1Base hydrolysis of the ester bonds by intracellular esterases releases the acetate groups and the resulting compound, 5(6)-carboxy-2',7',-dichlorofluorescein, is oxidized by reactive oxygen species (ROS) and nitric oxide (NO) and displays excitation/emission maxima of 504 and 530 nm, respectively, which can be quantified as a measure of oxidant production.
Chemical Properties
Molecular Weight529.3
FormulaC25H14Cl2O9
Cas No.127770-45-0
SmilesCC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C3(OC(=O)c4cc(ccc34)C(O)=O)c2cc1Cl.CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C3(OC(=O)c4ccc(cc34)C(O)=O)c2cc1Cl
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
DMF: 30 mg/mL (56.68 mM), Sonication is recommended.
DMSO: 30 mg/mL (56.68 mM), Sonication is recommended.
Solution Preparation Table
DMF/DMSO
1mg5mg10mg50mg
1 mM1.8893 mL9.4464 mL18.8929 mL94.4644 mL
5 mM0.3779 mL1.8893 mL3.7786 mL18.8929 mL
10 mM0.1889 mL0.9446 mL1.8893 mL9.4464 mL
20 mM0.0945 mL0.4723 mL0.9446 mL4.7232 mL
50 mM0.0378 mL0.1889 mL0.3779 mL1.8893 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

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