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5(6)-TAMRA SE

🥰Excellent
Catalog No. T18898Cas No. 246256-50-8
Alias 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester

5(6)-TAMRA SE (5(6)-Carboxytetramethylrhodamine N-succinimidyl ester) is the amine-reactive, mixed isomer form of TAMRA. 5(6)-TAMRA SE is a dye for oligonucleotide labeling and automated DNA sequencing applications.

5(6)-TAMRA SE

5(6)-TAMRA SE

🥰Excellent
Purity: 95%
Catalog No. T18898Alias 5(6)-Carboxytetramethylrhodamine N-succinimidyl esterCas No. 246256-50-8
5(6)-TAMRA SE (5(6)-Carboxytetramethylrhodamine N-succinimidyl ester) is the amine-reactive, mixed isomer form of TAMRA. 5(6)-TAMRA SE is a dye for oligonucleotide labeling and automated DNA sequencing applications.
Pack SizePriceAvailabilityQuantity
5 mg$50In Stock
10 mg$61In Stock
25 mg$97In Stock
50 mg$156In Stock
100 mg$249In Stock
1 mL x 10 mM (in DMSO)$68In Stock
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Purity:95%
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Product Introduction

Bioactivity
Description
5(6)-TAMRA SE (5(6)-Carboxytetramethylrhodamine N-succinimidyl ester) is the amine-reactive, mixed isomer form of TAMRA. 5(6)-TAMRA SE is a dye for oligonucleotide labeling and automated DNA sequencing applications.
Cell Research
Instructions
I. Reagent preparation: Select DMSO (e.g. 10 mM stock solution) or water, and adjust the specific concentration according to the experimental requirements. Generally, a dye concentration of 1–10 μM is used for labeling.
II. Operation steps
1. Labeling process:
1) Prepare oligonucleotides: Select appropriate oligonucleotides and dissolve them, with a common concentration of 10–100 μM.
Note: Ensure that the oligonucleotides are of good quality and are synthesized according to the experimental requirements.
2. Labeling reaction:
1) Add 5(6)-TAMRA SE solution to the oligonucleotide solution. A molar ratio of 1:10 to 1:100 is usually used.
2) The reaction is usually carried out within 30 minutes to 1 hour, and the reaction conditions are room temperature or slightly lower temperature (such as 4°C).
3) The reaction time and temperature can be optimized according to the needs of the experiment.
3. Reaction optimization:
1) The reaction can be carried out in phosphate buffered saline (PBS) or Tris buffer, maintaining the pH value between 7.0 and 8.5, which is helpful for labeling reaction.
2) If necessary, appropriate amount of chemical reagents (such as esterase or amino acid to prevent non-specific reaction) can be added to the reaction to optimize the labeling effect.
4. Removal of unbound dye:
1) After the reaction, unbound 5(6)-TAMRA SE needs to be removed. This can be done by the following methods:
2) Dialysis: Remove free dye in the solution by dialysis.
3) Gel filtration: Use gel filtration column to remove unbound dye.
4) Centrifugation: Separate the labeled oligonucleotide from the free dye by centrifugation.
5. Fluorescence detection: The labeled oligonucleotide can be detected by fluorescence. Measure the fluorescence signal using a fluorescence microscope, real-time PCR instrument, or other fluorescence imaging system:
Excitation wavelength: 540 nm
Emission wavelength: 595 nm
6. Data analysis:
Based on the fluorescence intensity of the marker, the characteristics of the target DNA sequence, the binding efficiency of the probe, and the application of oligonucleotides in DNA sequencing can be analyzed.
Notes:
1. Solubility: 5(6)-TAMRA SE has good solubility, but it is necessary to avoid exposing the dye to strong light during use to prevent the fluorescence signal from attenuating.
2. Nonspecific binding: During the labeling reaction, the concentration and reaction time of the dye should be controlled to avoid nonspecific binding of the dye to non-target molecules.
3. Storage: The dye should be kept away from light and usually needs to be stored at low temperature (-20°C) to maintain its stability.
Alias5(6)-Carboxytetramethylrhodamine N-succinimidyl ester
Chemical Properties
Molecular Weight527.53
FormulaC29H25N3O7
Cas No.246256-50-8
SmilesCN(C)c1ccc2c(Oc3cc(ccc3C22OC(=O)c3cc(ccc23)C(=O)ON2C(=O)CCC2=O)N(C)C)c1.CN(C)c1ccc2c(Oc3cc(ccc3C22OC(=O)c3ccc(cc23)C(=O)ON2C(=O)CCC2=O)N(C)C)c1
Relative Density.no data available
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 25 mg/mL (47.39 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM1.8956 mL9.4781 mL18.9563 mL94.7813 mL
5 mM0.3791 mL1.8956 mL3.7913 mL18.9563 mL
10 mM0.1896 mL0.9478 mL1.8956 mL9.4781 mL
20 mM0.0948 mL0.4739 mL0.9478 mL4.7391 mL

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TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
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Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
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