Trimethylcolchicinic acid (N-Deacetylcolchiceine) is an orally available colchicine analog that binds to tubulin and inhibits its polymerization into microtubules, preventing cell division and having potential antitumor activity.

Trimethylcolchicinic acid exhibits strong antimitotic effects in vitro. When applied to cultured mouse lymphocytes and HeLa cells at a concentration of 1 × 10⁻⁷ M for 24 hours, caused disruption of spindle formation and blockage of mitosis, indicating microtubule inhibition similar to colchicine[1].

In vivo studies in mice demonstrated that intraperitoneal injection of Trimethylcolchicinic acid (0.5–2 mg/kg) produced pronounced antimitotic effects and disrupted nuclear division in bone marrow cells. After 3 days of treatment, the mitotic index was significantly reduced, indicating potent microtubule interference in vivo[1].