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JHU395

Name: JHU395
Brand: TargetMol
SKU: T11717
Price: 61 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T11717Cas No. 2079938-92-2

JHU395 is an orally-bioavailable prodrug of a lipophilic glutamine antagonist (GA), 6-diazo-5-oxo-L-norleucine (DON). It demonstrates delivery of DON to malignant peripheral nerve sheath tumor (MPNST) both in vitro and in vivo, resulting in significant antitumor activity in MPNST. Additionally, JHU395 exhibits plasma stability.

JHU395

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Purity: 99.57%

Catalog No. T11717Cas No. 2079938-92-2

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$61	-	In Stock
5 mg	$147	In Stock	In Stock
10 mg	$255	In Stock	In Stock
25 mg	$488	In Stock	In Stock
50 mg	$762	-	In Stock
100 mg	$1,160	-	In Stock
200 mg	$1,560	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products. If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

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Batch Information

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Purity:99.57%

ee:100%

Appearance:Oil

Color:Yellow

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COA ORP CSFC HPLC HNMR

Product Introduction

Bioactivity

Description	JHU395 is an orally-bioavailable prodrug of a lipophilic glutamine antagonist (GA), 6-diazo-5-oxo-L-norleucine (DON). It demonstrates delivery of DON to malignant peripheral nerve sheath tumor (MPNST) both in vitro and in vivo, resulting in significant antitumor activity in MPNST. Additionally, JHU395 exhibits plasma stability.
In vitro	JHU395 (1、10、100 μmol/L) inhibits the growth of multiple biosynthetic processes by tumor (MPNST) cells, while the growth of immortalized Schwann cells is minimally affected. JHU395 induces less PARP cleavage as a marker of apoptosis in human MPNST cells. JHU395 is a plasma-stable lipophilic GA prodrug that delivers DON to MPNST in an in vitro plasma-to-tumor cell partitioning assay measurement.[1]
In vivo	JHU395 (oral gavage; 1.2 mg/kg × 5 days, 0.5 mg/kg × 9 days) delivers active GA to tumors with over twofold higher tumor-to-plasma exposure and significantly inhibits tumor growth in a murine flank MPNST model without observed toxicity.[1]

Chemical Properties

Molecular Weight	447.48
Formula	C₂₂H₂₉N₃O₇
Cas No.	2079938-92-2
Smiles	C(OC(C(C)(C)C)=O)(OC(N[C@H](C(OC(C)C)=O)CCC(C=[N+]=[N-])=O)=O)C1=CC=CC=C1
Relative Density.	no data available

Storage & Solubility Information

Storage

Pure form: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 90 mg/mL (201.13 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Corn Oil: 3.3 mg/mL (7.37 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2347 mL	11.1737 mL	22.3474 mL	111.7368 mL
5 mM	0.4469 mL	2.2347 mL	4.4695 mL	22.3474 mL
10 mM	0.2235 mL	1.1174 mL	2.2347 mL	11.1737 mL
20 mM	0.1117 mL	0.5587 mL	1.1174 mL	5.5868 mL
50 mM	0.0447 mL	0.2235 mL	0.4469 mL	2.2347 mL
100 mM	0.0223 mL	0.1117 mL	0.2235 mL	1.1174 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc