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Citric acid

Name: Citric acid
Brand: TargetMol
SKU: T5S0636
Price: 38 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T5S0636Cas No. 77-92-9

Alias Citro, Citretten

Citric acid (Citro) is a weak organic tricarboxylic acid found in citrus fruits. Citric acid is a food additive and a natural preservative.

Citric acid

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Purity: 98.88%

Catalog No. T5S0636Alias Citro, CitrettenCas No. 77-92-9

Citric acid (Citro) is a weak organic tricarboxylic acid found in citrus fruits. Citric acid is a food additive and a natural preservative.

Pack Size	Price	USA Warehouse	Global Warehouse
500 mg	$38	In Stock	In Stock
1 g	$54	In Stock	In Stock
5 g	$129	-	In Stock
10 g	$189	-	In Stock
25 g	$319	-	In Stock
1 mL x 10 mM (in DMSO)	$44	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:98.88%

Appearance:Solid

Color:White

Resource Download

COA LCMS HNMR

Product Introduction

Citric acid AI Summary

Citric acid exhibits a range of bioactivities and interactions across various biological assays. It demonstrates significant binding energy, with a measured binding energy of 5.0 kCal mol-1, indicating the potential to interact with other molecules. The compound shows weak inhibitory activity against the SH2 domain of human p60 c-Src tyrosine kinase, with an IC50 value greater than 40,000,000.0 nM in a surface plasmon resonance assay. In enzymatic assays, Citric acid exhibits inhibitory activity against rat ATP-Citrate lyase, with a relative Vmax value of 1.0 and a Km value of 110,000.0 nM. It also shows binding affinity for Helicobacter pylori DHQase 2 (Kd of 2500.0 nM) and Streptomyces coelicolor DHQase 2 (Kd of 7300.0 nM), and inhibitory activity for Mycobacterium tuberculosis DHQase 2 at 20 mM with inhibition below 10.0%. The compound lowers plasma glucose levels by 47.3% in Swiss-Webster mice at a dose of 250 mg/kg intraperitoneally after 4 hours, although it also demonstrates toxicity with an LC50 of 50.0 ppm in brine shrimp. It inhibits both Bacillus licheniformis beta-lactamase BS3 and beta-lactamase TEM1 with Ki values of 490,000.0 nM and 730,000.0 nM, respectively, in an acetate buffer at pH 5. Furthermore, Citric acid shows 100.0% HDAC inhibitory activity at 500 uM in a fluorimetric assay and potency in various cellular ATP level measurements, ranging from 2.0 nM to 79,432.8 nM. It also demonstrates activity in receptor signaling pathways, such as glucocorticoid receptor and peroxisome proliferator-activated receptor gamma, and inhibits aldehyde dehydrogenase and lipase co-activator protein ABHD5 interactions. Additionally, it inhibits the uptake of estrone sulfate in OATP-B-expressing HEK293 cells with 97.7% activity and exhibits antioxidant properties with an EC50 of 0.074 ug/mL-1 for Fe2+ chelating activity. The compound also shows inhibition against human hexahistidine-tagged full-length FTO and recombinant ALKBH5 catalytic domain, with IC50 values of 300,000.0 nM and 488,000.0 nM, respectively. Citric acid has an LC50 of 50.0 ppm in brine shrimp toxicity assays and demonstrates a range of biochemical and hematological parameter effects, indicating potential hepatotoxic, nephrotoxic, and hematotoxic impacts. It shows antimicrobial activity against Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa, and Staphylococcus aureus, with inhibitory zone sizes ranging from 0.0 mm to 10.67 mm at 40 μg per disc. The compound also possesses antiviral activity, inhibiting SARS-CoV-2 induced cytotoxicity in Caco-2 and VERO-6 cells, as well as showing an IC50 of 212,600.0 nM against Paramecium bursaria chlorella virus 1 CPH. Finally, Citric acid exhibits inhibitory activity on various enzymes, including human ACMSD, HDAC6, PHD1, PHD2, PHD3, KDM5B, and N-terminal His6-tagged recombinant human ALKBH5 catalytic AlkB domain. It demonstrates a lack of significant binding affinity or inhibition in assays targeting DRD1, SLC6A3, ESR1, and other receptors and enzymes at explored concentrations (AC50 > 30000.0 nM). This extensive profile suggests potential therapeutic applications, but also highlights the need for further studies to comprehensively understand its bioactivity and safety profile..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Citric acid (Citro) is a weak organic tricarboxylic acid found in citrus fruits. Citric acid is a food additive and a natural preservative.
Targets&IC50	GF:0.28 per cent, HaCaT cells:12.5 mM
In vitro	METHODS: HaCaT cells were treated with Citric acid (0-12.5 mM) for 24 hours, and the percentage of viable cells was determined by flow cytometry. RESULTS: Citric acid exhibited anti-proliferative activity in a dose-dependent manner. [1] METHODS: HaCaT cells were treated with Citric acid (12.5 mM) for 48 hours, and the levels of related proteins were detected by western blot. RESULTS: Citric acid increased the expressions of FAS, BAX, BID, AIF, EndoG, cytochrome C, PARP, GADD153, GRP78 and Caspase-3, -8, -9, and decreased the expressions of BCL-2 and BCL-Xl. [1]
In vivo	METHODS: To study the effect of Citric acid on the liver of mice, Citric acid (120, 240, and 480 mg/kg) was intraperitoneally injected into mice. RESULTS: Citric acid significantly reduced the activity of GSH-Px in the liver of mice, induced an increase in the level of MDA (malondialdehyde), and increased the activity of caspase-3 to induce apoptosis in a dose-dependent manner. [2] METHODS: To study the nephrotoxicity of Citric acid, Citric acid (120, 240, and 480 mg/kg) was intraperitoneally injected into mice every week for 3 consecutive weeks. RESULTS: Citric acid causes nephrotoxicity in mice. [3]
Synonyms	Citro, Citretten

Chemical Properties

Molecular Weight	192.12
Formula	C₆H₈O₇
Cas No.	77-92-9
Smiles	OC(=O)CC(O)(CC(O)=O)C(O)=O
Relative Density.	1.67. Temperature:20 °C.

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 100 mg/mL (520.51 mM), Sonication is recommended.

DMSO: 240 mg/mL (1249.22 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (26.03 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	5.2051 mL	26.0254 mL	52.0508 mL	260.2540 mL
5 mM	1.0410 mL	5.2051 mL	10.4102 mL	52.0508 mL
10 mM	0.5205 mL	2.6025 mL	5.2051 mL	26.0254 mL
20 mM	0.2603 mL	1.3013 mL	2.6025 mL	13.0127 mL
50 mM	0.1041 mL	0.5205 mL	1.0410 mL	5.2051 mL
100 mM	0.0521 mL	0.2603 mL	0.5205 mL	2.6025 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc