Integrin alpha V beta 6 Protein, Human, Recombinant (Flag & His)

Integrin alpha-5, also known as ITGA5, is a single-pass type I membrane protein which belongs to the integrin alpha chain family. ITGA5 contains 7 FG-GAP repeats. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of ITGAV&ITGB6 via clathrin-mediated endocytosis promotes carcinoma cell invasion.

Pack Size	Availability	Price/USD
100 μg	In stock	$ 517.00
200 μg	5 days	$ 913.00
500 μg	5 days	$ 1,930.00

Pack Size

Availability

Price/USD

Quantity

100 μg

In stock

$ 517.00

200 μg

5 days

$ 913.00

500 μg

5 days

$ 1,930.00

Biological Information

Testing in progress

Description

Species	Human
Expression System	HEK293
Tag	Flag,His
Accession Number	P06756-1&P18564-1
Synonyms	Integrin alpha V β6, ITGAV & ITGB6, integrin, alpha 5 (fibronectin receptor, alpha polypeptide), Integrin α V β 6, integrin, α5 (fibronectin receptor, α polypeptide)
Construction	A DNA sequence encoding the extracellular domain (Met 1-Val992) of human ITGAV (P06756-1) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asn707) of human ITGB6 (P18564-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human ITGAV&ITGB6 heterodimer was purified.
Protein Purity	> 95 % as determined by SDS-PAGE
Molecular Weight	Approxiamtely 190.4 kDa

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Integrin alpha-5, also known as ITGA5, is a single-pass type I membrane protein which belongs to the integrin alpha chain family. ITGA5 contains 7 FG-GAP repeats. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of ITGAV&ITGB6 via clathrin-mediated endocytosis promotes carcinoma cell invasion.