Testing in progress

Integrin alpha V beta 6 Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 190.4 kDa and the accession number is P06756-1&P18564-1.

Human

HEK293 Cells

Integrin alpha V β6,ITGAV & ITGB6,integrin, alpha 5 (fibronectin receptor, alpha polypeptide),Integrin α V β 6,integrin, α5 (fibronectin receptor, α polypeptide)

The extracellular domain (Met 1-Val992) of Human ITGAV (P06756-1) was fused with a flag tag at the C-terminus, constructed the plasmid 1; The extracellular domain (Met1-Asn707) of Human ITGB6 (P18564-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Human ITGAV&ITGB6 heterodimer was purified.

> 95 % as determined by SDS-PAGE

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Integrin alpha-5, also known as ITGA5, is a single-pass type I membrane protein which belongs to the integrin alpha chain family. ITGA5 contains 7 FG-GAP repeats. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of ITGAV&ITGB6 via clathrin-mediated endocytosis promotes carcinoma cell invasion.