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Dehydrotrametenolic acid

Name: Dehydrotrametenolic acid
Brand: TargetMol
SKU: TN1086
Rating: 4.5 (1 reviews)

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Catalog No. TN1086Cas No. 6879-05-6

Alias Dehydroeburicoic acid

Dehydrotrametenolic acid (Dehydroeburicoic acid) induces necrotic cell death that involves Ca(2+) overload, mitochondrial dysfunction, and calpain activation in human glioblastomas.

Dehydrotrametenolic acid

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Catalog No. TN1086Alias Dehydroeburicoic acidCas No. 6879-05-6

Dehydrotrametenolic acid (Dehydroeburicoic acid) induces necrotic cell death that involves Ca(2+) overload, mitochondrial dysfunction, and calpain activation in human glioblastomas.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$326	7-10 days	7-10 days
5 mg	$897	7-10 days	7-10 days
1 mL x 10 mM (in DMSO)	$1,083	7-10 days	7-10 days

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Appearance:Solid

Color:White

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Product Introduction

Bioactivity

Description	Dehydrotrametenolic acid (Dehydroeburicoic acid) induces necrotic cell death that involves Ca(2+) overload, mitochondrial dysfunction, and calpain activation in human glioblastomas.
In vitro	3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase release assays indicated that Dehydroeburicoic acid inhibited the proliferation of the human glioblastoma cell U87MG. In addition, Annexin V and propidium iodide staining showed that Dehydroeburicoic acid treatment led to a rapid increase of glioblastomas in the necrotic/late apoptotic fraction, whereas cell cycle analysis revealed that Dehydroeburicoic acid failed to significantly enhance the population of U87MG cells in the hypodiploid (sub-G1) fraction. Using electron microscopy, we found that Dehydroeburicoic acid induced significant cell enlargements, massive cytoplasmic vacuolization, and loss of mitochondrial membrane integrity. Dehydroeburicoic acid treatment triggered an intracellular Ca(2+) increase, and Dehydroeburicoic acid-induced cell death was significantly attenuated by BAPTA-AM but not ethylenediaminetetraacetic acid or ethylene glycol tetraacetic acid. Dehydroeburicoic acid instigated a reduction of both mitochondrial transmembrane potential and intracellular ATP level. Moreover, Dehydroeburicoic acid induced proteolysis of alpha-spectrin by calpain, and Dehydroeburicoic acid cytotoxicity in U87MG cells was caspase-independent but was effectively blocked by calpain inhibitor. Interestingly, Dehydroeburicoic acid also caused autophagic response that was prevented by calpain inhibitor.
Synonyms	Dehydroeburicoic acid

Chemical Properties

Molecular Weight	468.71
Formula	C₃₁H₄₈O₃
Cas No.	6879-05-6
Smiles	[H][C@@]1(CC[C@@]2(C)C3=CC[C@@]4([H])C(C)(C)[C@@H](O)CC[C@]4(C)C3=CC[C@]12C)[C@@H](CCC(=C)C(C)C)C(O)=O
Relative Density.	1.07 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 10 mM, Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1335 mL	10.6676 mL	21.3352 mL	106.6758 mL
5 mM	0.4267 mL	2.1335 mL	4.2670 mL	21.3352 mL
10 mM	0.2134 mL	1.0668 mL	2.1335 mL	10.6676 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc