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BRL 54443

Name: BRL 54443
Brand: TargetMol
SKU: T2459
Price: 40 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2459Cas No. 57477-39-1

Alias BRL54443

BRL 54443 is a potent 5-HT1E/1F receptor agonist (pKi values: 8.7 and 8.9, respectively).

BRL 54443

🥰Excellent

Purity: 98.07%

Catalog No. T2459Alias BRL54443Cas No. 57477-39-1

BRL 54443 is a potent 5-HT1E/1F receptor agonist (pKi values: 8.7 and 8.9, respectively).

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$40	In Stock	In Stock
10 mg	$68	In Stock	In Stock
25 mg	$147	In Stock	In Stock
50 mg	$247	In Stock	In Stock
100 mg	$367	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$44	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Batch Information

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Purity:98.07%

Resource Download

COA LCMS HNMR HPLC

Product Introduction

Bioactivity

Description	BRL 54443 is a potent 5-HT1E/1F receptor agonist (pKi values: 8.7 and 8.9, respectively).
Targets&IC50	5-HT1A:7.2(pKi), 5-HT1D:7.2(pKi), 5-HT2A receptor:1259 nM (Ki), 5-HT2B receptor:100 nM (Ki), 5-HT1F:9.25(pKi), 5-HT7 receptor:>10,000 nM (Ki), 5-HT1E:8.7(pKi), 5-HT6 receptor:>10,000 nM (Ki), 5-HT1B:6.9(pKi), 5-HT2C receptor:316 nM (Ki)
In vitro	BRL 54443 induced contractions with a -logEC50 of 6.52. Contractions induced by BRL 54443 are likely mediated by stimulation of 5-HT 5-HT 2A receptors.
In vivo	BRL 54443 induced contractions with a -logEC50 of 6.52. Contractions induced by BRL 54443 are likely mediated by stimulation of 5-HT 5-HT 2A receptors.
Kinase Assay	In Vitro Radiometric PKD Kinase Assay: The radiometric kinase assay is carried out by coincubating 0.5 μCi of [γ-32P]ATP, 20 μM ATP, 50 ng of purified recombinant human PKD (PKD1, PKD2, and PKD3) or CAMKIIα proteins, and 2.5 μg of Syntide-2 in 50 μl of kinase buffer that contains 50 mM Tris-HCl, pH 7.5, 4 mM MgCl2, 10 mM β-mercaptoethanol. The reaction is carried out under conditions that the initial rate is within the linear kinetic range. The filter papers are then washed three times in 0.5% phosphoric acid, air-dried, and counted using a Beckman LS6500 multipurpose scintillation counter.
Synonyms	BRL54443

Chemical Properties

Molecular Weight	230.31
Formula	C₁₄H₁₈N₂O
Cas No.	57477-39-1
Smiles	OC=1C=C2C(=CNC2=CC1)C3CCN(C)CC3
Relative Density.	1.196 g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: 2 mg/mL (8.68 mM), Sonication is recommended.

DMSO: 43 mg/mL (186.7 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (8.68 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	4.3420 mL	21.7099 mL	43.4197 mL	217.0987 mL
5 mM	0.8684 mL	4.3420 mL	8.6839 mL	43.4197 mL

DMSO

	1mg	5mg	10mg	50mg
10 mM	0.4342 mL	2.1710 mL	4.3420 mL	21.7099 mL
20 mM	0.2171 mL	1.0855 mL	2.1710 mL	10.8549 mL
50 mM	0.0868 mL	0.4342 mL	0.8684 mL	4.3420 mL
100 mM	0.0434 mL	0.2171 mL	0.4342 mL	2.1710 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc