5(and6)carboxy snarf1

5-(6)-Carboxy RhodFluor is a cell-impermeant pH indicator.

bis-ANS is a high-affinity non-covalent extrinsic fluorescent dye used to analyze protein conformation.[1] Its predominant interaction with proteins is through its hydrophobic phenyl and naphthyl rings.[2] bis-ANS has an excitation maxima of 390 nm.[3] It has an emission maximum of 523 nm when free in solution but undergoes a blue shift with an increase in fluorescence intensity when bound to protein; for example, when bound to intestinal fatty acid binding protein (FAPB2) it has emission maxima of 484-496 nm. bis-ANS has been used to label mechanically damaged neurons in acute brain slices.4 It also potently inhibits microtubule assembly.[5],[6]

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate is an oxidant-sensitive and cell-permeable fluorescent probe.1Base hydrolysis of the ester bonds by intracellular esterases releases the acetate groups and the resulting compound, 5(6)-carboxy-2',7',-dichlorofluorescein, is oxidized by reactive oxygen species (ROS) and nitric oxide (NO) and displays excitation emission maxima of 504 and 530 nm, respectively, which can be quantified as a measure of oxidant production. 1.Hempel, S.L., Buettner, G.R., O'Malley, Y.Q., et al.Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: Comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123Free Radic. Biol. Med.27(1-2)146-159(1999)

Biotin-11-dUTP [tetra(triethylammonium) salt] [(2S,3S,4R,5R,6R)-5-(2,4-dioxopyrimidin-1-yl)-4-hydroxy-6-(hydroxymethyl)-2-[(3aR,4S,6R,6aS)-2-oxohexahydro-2H-thieno[3,4-d]imidazol-4-yl]-tetrahydro-2H-furo[3,2-d][1,3,2]dioxaphosphorin-3-yl]phosphonate] is used as a fluorescent substitute for dTTP.

HKSOX-1r (5 6-mixture) is a fluorescent probe specifically designed for the imaging and detection of endogenous superoxide in live cells and in vivo applications, demonstrating superior selectivity and sensitivity to the superoxide anion radical [1]. For storage, it must be protected from light.

Flutax-2 (5 6-mixture), an active fluorescent derivative of paclitaxel, binds to polymerized α,β tubulin dimers, with an excitation emission wavelength of 496 524 nm. Derived from paclitaxel, a diterpenoid secondary metabolite from Taxus species utilized in various cancer research [1], it requires storage protection from light.

HKSOX-1m hydrobromide (5 6-mixture), a mitochondria-targeted O2•− fluorescent probe (Ex Em=509 534nm; green), demonstrates exceptional selectivity and sensitivity for O2•− across a wide pH spectrum, robustly distinguishing it from strong oxidants and prevalent cellular reductants [1].

Mca-(endo-1α-Dap(Dnp))-TNF-α(-5 to +6) amide (human) TFA is a peptide that functions as a fluorescence resonance energy transfer (FRET)-based substrate, with its activity determined by the change in fluorescence intensity following cleavage [1].

5'6-FAM-GGUGAAAGACGAUGGACAATT,UUGUCCAUCGUCUUUCACCTT is a double-stranded ribonucleoside modified with the fluorescent molecule 5'6-FAM, a combination of 5-FAM and 6-FAM, exhibiting excitation emission wavelengths at 490 nm 520 nm [1].

(5)6-Carboxytetramethylrhodamine, a bright orange-fluorescent dye with absorption emission maxima of ~555 580 nm [1], contains a carboxylic acid that reacts with primary amines via carbodiimide activation.

5(6)-Carboxy-2',7'-dichlorofluorescein diacetate N-succinimidyl ester (cDCFDASE) serves as a fluorescent probe for assessing the intracellular pH (pH in) in living cells under various external pH conditions (pH ex) [1]. This compound is utilized to measure pH dynamics within cells, facilitating critical insights into cellular function and behavior.

5(6)-Carboxy-2',7'-dichlorofluorescein serves as an excellent substrate for the MRP2 vesicular transport assay, offering superior detection and transport characteristics [1].

5(6)-Carboxyfluorescein diisobutyrate, a protected form of 5(6)-carboxyfluorescein, serves as a fluorescence pH indicator [1].

5(6)-Carboxynaphthofluorescein diacetate serves as a fluorescent substrate in esterase assays, where it is cleaved by intracellular esterases, resulting in red fluorescence for enzyme activity measurement (Ex = 590 nm; Em = 645 nm) [1].

5(6)-Carboxynaphthofluorescein, a pH-dependent fluorophore, exhibits high sensitivity in alkaline pH settings, making it suitable for the development of fiber-optic pH sensors. Additionally, it serves as a fluorescent pH indicator with excitation emission wavelengths of 593 668 nm [1].

5(6)-SFX, SE (Compound SFX-SE) is a fluorescent dye used for labeling primary amines and nascent proteins [1] [2].

6-TET phosphoramidite (5′-Tetrachlorofluorescein phosphoramidite) serves as a fluorescent dye to label oligonucleotides with fluorescein [1].

CelRed nucleic acid gel stain 10,000× concentrate in water is a fluorescent dye used for nucleic acid staining, suitable for staining and detecting dsDNA, ssDNA, or RNA in agarose gels or polyacrylamide gels.

The cell-permeant pH indicator, carboxy SNARF-1, acetoxymethyl ester, acetate (CAS #126208-13-7) has a pKa of ~7.5 after de-esterification, thus is useful for measuring pH changes between pH 7 and pH 8. Carboxy-SNARF-1 exhibits a significant pH-dependent