detection

AQC (6-Aminoquinolyl-N-hydroxysccinimidyl carbamate) is a reagent used in HPLC for the fluorescence detection of amino acid or protein sequences. It reacts with primary and secondary amino acids to produce fluorescent derivatives, enabling the detection of amino acids at picomolar levels.

CFSE (CFDA-SE) is a fluorescent dye with cell membrane permeability. CFSE irreversibly binds to intracellular proteins in living cells and is used for the detection of cell proliferation. The labeled cells fluoresce in green color with excitation wavelength of 488 nm and emission wavelength of 518 nm.

MitoSOX Red is a fluorescent dye serving as a mitochondria-targeted fluorescent probe for superoxide, with cell membrane permeability and specificity. Upon oxidation, MitoSOX Red subsequently binds to nucleic acids within mitochondria/nuclei, producing intense red fluorescence, and is employed for detecting mitochondrial superoxide in live cells.

GLF16 FA is a fluorescent SBB analogue for rapid detection of senescent cells using flow cytometry and live cell sorting.

Msr-Ratio (Msr-green) (Msr-green) is a ratiometric fluorescent probe specifically designed for the detection of methionine sulfoxide reductase (MSR) activity. It has an excitation wavelength of 375 nm and an emission wavelength of 550 nm. This probe offers the ability to monitor the enzyme activity both in vitro and in live cells, making it a valuable tool for studying MSR function.

GLF16 is a hydrophilic fluorescent SBB analog and robustly identifies senescence. It enables the use of flow cytometry and live cell sorting for rapid senescent cell detection. GLF16 is suitable for in vivo identification, isolation, and downstream characterization of senescent cell populations at the single-cell level in various biological materials.

Lucifer Yellow CH dipotassium is a fluorescent dye, a water-soluble biological tracer (Ex/Em = 430/540 nm) containing a free hydrazino group that can react with aldehydes, used for neuronal tracing and gap junction detection.

Evans blue (C.I. 23860) is an effective inhibitor of glutamate uptake by synaptic vesicles and also acts as an antagonist of AMPA/kainate receptors. Evans blue is a water-soluble azo fluorescent dye that binds to serum albumin and is used to detect vascular permeability, blood-brain barrier integrity, and permeability.

H2DCFDA (DCFH-DA) belongs to the class of green fluorescent dyes and is a probe for the detection of intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm) with cell membrane permeability.

2,3-Diaminonaphthalene is a highly selective fluorometric and colorimetric reagent for selenium detection, and it is also used for the fluorometric determination of nitrite.

ABTS diammonium salt (AzBTS-(NH₄)₂) is a substrate for horseradish peroxidase (HRP). ABTS diammonium salt is a water-soluble redox chromogenic probe that, upon oxidation, generates a stable blue-green cationic radical (ABTS⁺·). It exhibits characteristic absorption peaks at 405 nm, 414 nm, 645 nm, and 734 nm (with 734 nm commonly used as the detection wavelength) and serves as a classic tool for assessing antioxidant activity, peroxidase activity, and free radical scavenging capacity.

BCIP (BCIP p-toluidine salt) (BCIP p-toluidine salt) is an artificial chromogenic substrate. It is used for the sensitive colorimetric detection of alkaline phosphatase activity.

Nile Red ((Nile Blue A oxazone)) is a lipophilic, environment-sensitive fluorescent dye. Highly hydrophobic, with fluorescence significantly enhanced in lipid-rich environments. Commonly used for specific staining of intracellular lipid droplets, with excitation/emission wavelengths of 559/635 nm.

Reactive Blue 4, an anthraquinone dye, exhibits phytotoxic, cytotoxic, and genotoxic properties. It functions as a single colorimetric chemosensor for the sequential detection of multiple analytes, each with distinct optical responses, in aqueous media.

TMB dihydrochloride (Sure Blue TMB dihydrochloride) is an enzyme-linked immunosorbent assays (ELISA) and an excellent colorimetric substrate for the detection of horseradish peroxidase (HRP)-labeled probes used in staining procedures immunohistochemistry.

TMB (BM blue) is a chromogenic substrate used in staining procedures in immunohistochemistry and as a visualizing reagent in ELISA.

1-Naphthyl acetate is usually used in a rapid staining method for identification of macrophages. 1-Naphthyl acetate is a potent chromogenic substrate for the detection of erythrocyte acetylcholinesterase (AChE) activity. It has the potential to detect organophosphorus pesticide poisoning.

D-Luciferin Sodium is a specific natural substrate for firefly luciferase that catalyzes the production of characteristic yellow-green light in bioluminescent insects. It serves as a core tool for in vivo imaging, cell-level reporter gene detection, and analysis of luciferase activity in tissues and living organisms. The luciferase (luc) gene is a commonly used reporter gene for research and active molecule screening. Chemiluminescence technology is essentially background-free, making the luc reporter gene an ideal choice for detecting low levels of gene expression. Luciferase activity as low as 0.02 pg can be reliably measured in a standard fluorometer.

Indigo carmine (NSC-8646) is an indolesulfonic acid. It is used as a dye in renal function testing for the detection of nitrates and chlorates.

2',7'-Dichlorofluorescein diacetate (Diacetyldichlorofluorescein), a cell-permeable fluorogenic probe, is useful for the detection of nitric oxide (NO) and reactive oxygen species (ROS) and for the determination of the degree of overall oxidative stress.

Acridine Orange base is a cell-permeable fluorescent dye that binds to nucleic acids and emits different colors of fluorescence, which exhibits green fluorescence when bound to dsDNA (Ex=488 nm, Em=520-524 nm) and red fluorescence when bound to ssDNA or ssRNA (Ex=457 nm, Em=630-644 nm) and can be used for cell cycle analysis and apoptosis detection.