Ultra-Sensitive ECL Kit

Taking 100 mL packing for example:

1.High sensitivity

2.Strong signal output

3.Stable and long-lasting luminescence

4.Excellent reproducibility

Suitable for chemiluminescent detection of HRP-conjugated secondary antibodies in Western Blot assays, enabling high-sensitivity detection of extremely low-abundance protein signals.

1. Preparation of Working Solution

Prepare the ECL working solution by mixing equal volumes of Solution A and Solution B and gently invert to mix thoroughly. Keep the working solution at room temperature and protect from light before use. The prepared working solution remains stable for approximately 2 hours.

2. Membrane Handling

After secondary antibody incubation and thorough washing in the Western Blot procedure, carefully remove the membrane using flat-tip forceps. Gently blot excess liquid with absorbent paper (avoid contact between the paper and the protein-binding surface of the membrane). Then place the membrane flat on clean plastic wrap.

3. Substrate Incubation

Calculate the required volume based on membrane area and add approximately 0.1 mL of ECL working solution per square centimeter. Evenly dispense the solution onto the membrane surface to ensure complete coverage, and incubate at room temperature for 1–2 minutes.

4. Pre-imaging Preparation

After incubation, remove the membrane and discard excess working solution. Gently blot off residual liquid with absorbent paper. Place the membrane between two layers of plastic wrap and prepare it for X-ray film exposure or chemiluminescent imaging.

5. Imaging and Detection

For detection using a chemiluminescence imaging system, place the membrane into the instrument and set the appropriate parameters according to the imaging system’s operating instructions to acquire the signal.

Store at 4 ℃, protected from light, for 1 year.

1.When pipetting Solution A and Solution B, always use separate pipette tips. Do not mix or cross-contaminate the solutions, as this may cause gradual inactivation of Solution A or Solution B and negatively affect subsequent experimental results.

2.After use, immediately tighten the caps of all reagent bottles to prevent reagent degradation caused by exposure to air.

3.This product provides long-lasting chemiluminescence; more than 80% of the signal intensity is retained after 60 minutes. It is recommended to take full advantage of the strong signal within the first 60 minutes for film exposure or imaging.

4.If this product is overly sensitive and interferes with detection, substrates with lower sensitivity may be used instead, such as Sensitive ECL Kit (C2001) or Highly Sensitive ECL Kit (C2002).

5.The product is for R&D use only, not for diagnostic procedures, food, drug, household or other uses.

6.Please wear a lab coat and disposable gloves.

• C2003-Instruction Manual(255 KB)