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AMC-GlcNAc (compound 1) serves as a potent fluorogenic probe for the detection and characterization of β-hexosaminidase enzyme activity, offering continuous monitoring through turn-on fluorescence. Its fluorescence signal remains stable across a broad pH spectrum (Ex=325 nm, Em=390 nm). This compound facilitates ratiometric fluorescence detection with heightened sensitivity and minimal background, suitable for screening recombinant dispersin B activity in E. coli cell lysate [1].
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 5 mg | Inquiry | Inquiry | Inquiry | |
| 50 mg | Inquiry | Inquiry | Inquiry |
| Description | AMC-GlcNAc (compound 1) serves as a potent fluorogenic probe for the detection and characterization of β-hexosaminidase enzyme activity, offering continuous monitoring through turn-on fluorescence. Its fluorescence signal remains stable across a broad pH spectrum (Ex=325 nm, Em=390 nm). This compound facilitates ratiometric fluorescence detection with heightened sensitivity and minimal background, suitable for screening recombinant dispersin B activity in E. coli cell lysate [1]. |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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