GLF16 FA is a fluorescent SBB analogue for rapid detection of senescent cells using flow cytometry and live cell sorting.

Instructions
I. Solution preparation
1. Stock solution: Dissolve GLF16 FA in an appropriate solvent, usually anhydrous DMSO, to prepare a stock solution with a concentration of 1–10 mM.
2. Working solution: Dilute the stock solution to a working concentration, usually 1–5 µM, using an experimental buffer (such as PBS, pH 7.4) or culture medium.
II. Operation steps
Cell culture experiment
(1) Cell type: GLF16 FA can be used to detect senescent cells, especially for a variety of cell lines cultured in vitro.
(2) Treatment method: Add GLF16 FA to the cell culture medium and culture the cells for 30 minutes to 1 hour at 37°C, away from light.
(3) Cell function detection:
a. Senescence marker detection: Detect the fluorescence intensity of GLF16 FA in cells by flow cytometry or live cell sorting to identify senescent cells.
b. Cell survival rate detection: CCK-8 or other cell activity detection methods can be used to evaluate cell survival rate.
c. Senescence marker detection: Combined with other senescence markers, such as SA-β-gal, the characteristics of senescent cells can be further verified.
Animal experiments
(1) Treatment method: GLF16 FA is administered to experimental animals by intraperitoneal injection or tail vein injection. The dose is usually 1–10 mg/kg depending on the experimental design.
(2) Senescence detection: After injection, the fluorescence signal of senescent cells in vivo is detected by flow cytometry or other imaging techniques.
3. Calibration and control
(1) Control group: Set up an untreated cell or animal group as a control to verify the effect of GLF16 FA.
(2) Standard curve: Use senescent cell samples with known concentrations to establish a standard curve between the fluorescence signal and the proportion of senescent cells.
Notes
(1) Storage conditions: GLF16 FA should be stored at -20°C in the dark and avoid repeated freezing and thawing.
(2) Fluorescence detection: The excitation wavelength is usually 485–495 nm and the emission wavelength is 515–525 nm.
(3) Photosensitivity: GLF16 FA is light-sensitive and strong light exposure should be avoided during the experiment.
(4) Solubility: Ensure that GLF16 FA is completely dissolved during dissolution to avoid any solid residue.

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.

H2O: < 1 mg/mL (insoluble)

DMSO: 100 mg/mL (76.6 mM), Sonication is recommended.