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Z-LLE-AMC

Name: Z-LLE-AMC
Brand: TargetMol
SKU: T37417
Price: 40 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: Z-Leu-Leu-Glu-AMC) Copy Product Info

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Synonyms: Z-Leu-Leu-Glu-AMC

Catalog No. T37417 Copy Product Info

Purity: 99.47%

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Z-LLE-AMC (Z-Leu-Leu-Glu-AMC) is a fluorogenic substrate used to detect caspase-like post-glutamate peptide hydrolase activity in the 26S proteasome or 20S proteolytic core. Caspase-like activity can be quantified by fluorescent detection of free AMC (7-amino-4-methylcoumarin), which has an excitation wavelength of 340–360 nm and an emission wavelength of 440–460 nm. Z-LLE-AMC is typically used in cell lysates after experimental treatment. Additionally, Z-LLE-AMC can also be used in Parkinson's disease research.

Cas No. 348086-66-8

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Pack Size	Price	USA Stock	Global Stock
1 mg	$40	-	In Stock
5 mg	$106	-	In Stock
10 mg	$189	-	In Stock
25 mg	$378	-	In Stock
50 mg	$595	-	In Stock
100 mg	$862	-	In Stock
1 mL x 10 mM (in DMSO)	$156	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.47%

Appearance:Solid

Color:White

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COA MS HPLC

Product Introduction

Bioactivity

Description	Z-LLE-AMC (Z-Leu-Leu-Glu-AMC) is a fluorogenic substrate used to detect caspase-like post-glutamate peptide hydrolase activity in the 26S proteasome or 20S proteolytic core. Caspase-like activity can be quantified by fluorescent detection of free AMC (7-amino-4-methylcoumarin), which has an excitation wavelength of 340–360 nm and an emission wavelength of 440–460 nm. Z-LLE-AMC is typically used in cell lysates after experimental treatment. Additionally, Z-LLE-AMC can also be used in Parkinson's disease research.
In vitro	Method: In vitro, purified recombinant 20S proteasome was incubated with Z-LLE-AMC (75 μM) as a substrate to measure the post-acidic (postacidic)-like hydrolytic activity of the proteasome. Result: Under control conditions, the post-acidic-like activity of the purified 20S proteasome was 5.0±0.96 FU/min per mg. In the presence of 4 mM L-DOPA, the activity was 4.8±0.4 FU/min per mg, showing no significant difference from the control. In the presence of 10 μM lactacystin, the activity decreased to 0.75±0.075 FU/min per mg, representing an inhibition of approximately 85% (P<0.01) [1].
Synonyms	Z-Leu-Leu-Glu-AMC

Chemical Properties

Molecular Weight	664.76
Formula	C₃₅H₄₄N₄O₉
Cas No.	348086-66-8
Smiles	CC=1C=2C(=CC(NC([C@@H](NC([C@@H](NC([C@@H](NC(OCC3=CC=CC=C3)=O)CC(C)C)=O)CC(C)C)=O)CCC(O)=O)=O)=CC2)OC(=O)C1
Sequence	Cbz-Leu-Leu-Glu-AMC
Sequence Short	LLE

Storage & Solubility Information

Storage

Keep away from moisture, Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (120.34 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.5043 mL	7.5215 mL	15.0430 mL	75.2151 mL
5 mM	0.3009 mL	1.5043 mL	3.0086 mL	15.0430 mL
10 mM	0.1504 mL	0.7522 mL	1.5043 mL	7.5215 mL
20 mM	0.0752 mL	0.3761 mL	0.7522 mL	3.7608 mL
50 mM	0.0301 mL	0.1504 mL	0.3009 mL	1.5043 mL
100 mM	0.0150 mL	0.0752 mL	0.1504 mL	0.7522 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc