WSP-1 is a selective 2-pyridyl disulfide fluorescent probe for hydrogen sulfide detection and bioimaging.

1. Hydrogen sulfide detection
Experimental steps:
1. Prepare WSP-1 solution: WSP-1 is usually dissolved in an appropriate solvent (such as DMSO) and then mixed with cell culture or biological samples. Its concentration is usually between 1-5 μM (can be adjusted according to experimental requirements).
2. Incubate the cell/tissue sample: Add WSP-1 solution to live cells or tissues, usually 30-60 minutes, allowing the probe to enter the cells and react with hydrogen sulfide.
3. Fluorescence imaging: Use a fluorescence microscope or imaging system to monitor the fluorescence signal. WSP-1 emits fluorescence at a specific wavelength in the presence of hydrogen sulfide, usually within the green fluorescence range.
4. Data analysis: According to the fluorescence intensity, the concentration of hydrogen sulfide in cells or tissues can be quantitatively analyzed.
2. Application in cell and animal models
Experimental steps:
1. Preparation of cell/animal model: Prepare appropriate cell lines or animal models, inject or add WSP-1 probes through cell culture.
2. Monitoring and imaging: Use a fluorescence microscope or multiphoton microscope to capture fluorescence images at appropriate time points to analyze the dynamic changes of hydrogen sulfide.
3. Results analysis: The concentration of hydrogen sulfide and its relationship with pathological status were evaluated through fluorescence images and quantitative analysis.
3. Research on hydrogen sulfide:
Experimental steps:
1. Drug intervention or mutation model: Change hydrogen sulfide levels in cells or animals by adding synthesizers or inhibitors of hydrogen sulfide (such as inhibitors of certain hydrogen sulfide-generating enzymes).
2. Fluorescence monitoring: Monitor the changes in the fluorescence signal generated by WSP-1 to evaluate the concentration changes of hydrogen sulfide under different experimental conditions.
3. Results analysis: Fluorescence signals are used to quantitatively analyze the relationship between hydrogen sulfide and related physiological or pathological events (such as heart disease, neurodegenerative diseases, etc.).
Notes:
1. Solubility and stability: WSP-1 has good solubility, but when used, it should avoid direct contact with strong reducing agents or high concentrations of hydrogen sulfide to avoid affecting its performance.
2. Background interference: Background fluorescence in certain cells or tissues may interfere with measurements. Imaging conditions should be optimized and controlled experiments should be conducted when used.
3. Cell permeability: WSP-1 has a high permeability and can directly enter the cells and react with endogenous hydrogen sulfide, but for some cell types, it may be necessary to optimize experimental conditions.