Hoechst 33342 Staining Solution

Hoechst 33342 is a blue fluorescent dye that can stain the cell nucleus and penetrate the cell membrane. The fluorescence of Hoechst 33342 in its free state is weak or almost non-existent (with a maximum excitation wavelength of 346 nm and a maximum emission wavelength of 460 nm). When Hoechst 33342 dye binds to the A-T rich region of double stranded DNA, it can emit blue fluorescence under ultraviolet excitation (maximum excitation wavelength of 350 nm, maximum emission wavelength of 461 nm). Living cells have a certain ability to uptake Hoechst 33342 dye, while apoptotic cells increase their uptake of the dye and emit higher fluorescence intensity due to changes in cell membrane permeability. From this, live cells, apoptotic cells, and necrotic cells can be analyzed by detecting fluorescence intensity. Hoechst 33342 fluorescence staining is commonly used for cell apoptosis detection, cell cycle analysis, and cell viability identification.