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Fluorescein di-beta-D-galactopyranoside
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Catalog No. T18993Cas No. 17817-20-8
Alias Fluorescein di(β-D-galactopyranoside), FDG
Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λem=535 nm, λex=485 nm).
Fluorescein di-beta-D-galactopyranoside
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Purity: 99.02%
Catalog No. T18993Alias Fluorescein di(β-D-galactopyranoside), FDGCas No. 17817-20-8
Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λem=535 nm, λex=485 nm).
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 1 mg | $71 | - | In Stock | |
| 5 mg | $252 | - | In Stock | |
| 10 mg | $396 | - | In Stock |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Purity:99.02%
Appearance:Solid
Color:Yellow
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λem=535 nm, λex=485 nm). |
| In vitro | Fluorescein di(β-D-galactopyranoside) exhibits time- and dose-dependent increases in fluorescence in Hs68 cells, with fluorescence levels generated by the dual-substrate method significantly lower than those produced by Fluorescein di(β-D-galactopyranoside)[1]. |
| Cell Research | Procedure 1. After the cells have been cultured to the appropriate state, add 2 mM Fluorescein di-beta-D-galactopyranoside to each well after adding an aliquot of the reaction buffer and store the plate at 37°C in the dark for 24 hours without supply of CO2. 2. Observe and capture images using a fluorescence microscope. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Synonyms | Fluorescein di(β-D-galactopyranoside), FDG |
| Molecular Weight | 656.59 |
| Formula | C32H32O15 |
| Cas No. | 17817-20-8 |
| Smiles | OC[C@H]1O[C@@H](Oc2ccc3c(Oc4cc(O[C@@H]5O[C@H](CO)[C@H](O)[C@H](O)[C@H]5O)ccc4C33OC(=O)c4ccccc34)c2)[C@H](O)[C@@H](O)[C@H]1O |
| Relative Density. | 1.74 g/cm3 |
| Storage | keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||
| Solubility Information | DMSO: 5 mg/mL (7.62 mM), Sonication is recommended. | |||||||||||||||
Solution Preparation Table | ||||||||||||||||
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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