Drug Affinity Responsive Target Stability (DARTS) Assay Kit

The DARTS Assay Kit, based on the classic DARTS (Drug Affinity Responsive Target Stability) technology, is an experimental method that can verify the direct binding of small molecule drugs to target proteins without the need for compound modification. This technology was first proposed by Lomenick et al. in 2009 and is currently a sensitive, reliable, and easy to operate method for drug target discovery and mechanism of action research.
This kit provides the key buffer and protease treatment system required for DARTS experiments, which can efficiently and sensitively detect the binding characteristics of compounds and target proteins. This method does not require compound modification or labeling, and the experimental process is simple and the results are intuitive. It can be combined with subsequent Western blot, LC-MS/MS, or proteomic analysis to provide an efficient and reliable experimental solution for drug development, target discovery, and mechanism research.