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APF
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Catalog No. T38099Cas No. 359010-70-1
APF is an aromatic amino-fluorescein derivative that serves as a highly specific fluorescent probe for detecting highly reactive radicals, exhibiting low intrinsic fluorescence but converting into strongly fluorescent fluorescein upon oxidation by hydroxyl radicals, hypochlorite ions, and selected peroxidase intermediates. APF remain unreactive to nitric oxide and hydrogen peroxide, while with fluorescein displays excitation and emission maxima of 490 nm and 515 nm, respectively, enabling sensitive visualization of oxidative stress in biological systems.
APF
Copy Product Info😃Good
Catalog No. T38099Cas No. 359010-70-1
APF is an aromatic amino-fluorescein derivative that serves as a highly specific fluorescent probe for detecting highly reactive radicals, exhibiting low intrinsic fluorescence but converting into strongly fluorescent fluorescein upon oxidation by hydroxyl radicals, hypochlorite ions, and selected peroxidase intermediates. APF remain unreactive to nitric oxide and hydrogen peroxide, while with fluorescein displays excitation and emission maxima of 490 nm and 515 nm, respectively, enabling sensitive visualization of oxidative stress in biological systems.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 500 μg | $204 | 35 days | 35 days | |
| 1 mg | $326 | 35 days | 35 days | |
| 5 mg | Inquiry | 35 days | 35 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | APF is an aromatic amino-fluorescein derivative that serves as a highly specific fluorescent probe for detecting highly reactive radicals, exhibiting low intrinsic fluorescence but converting into strongly fluorescent fluorescein upon oxidation by hydroxyl radicals, hypochlorite ions, and selected peroxidase intermediates. APF remain unreactive to nitric oxide and hydrogen peroxide, while with fluorescein displays excitation and emission maxima of 490 nm and 515 nm, respectively, enabling sensitive visualization of oxidative stress in biological systems. |
| In vitro | In cellular assays for detecting Reactive Oxygen Species (ROS), APF was utilized at a working concentration of 1-10 μM. The probe effectively stained both suspension and adherent cells when incubated at 37°C for 20-60 minutes in serum-free media or PBS. This staining allowed for subsequent detection via fluorescence microscopy or flow cytometry [1]. |
| Molecular Weight | 423.42 |
| Formula | C26H17NO5 |
| Cas No. | 359010-70-1 |
| Smiles | O=C1OC2(C3=CC=C(O)C=C3OC4=CC(OC5=CC=C(N)C=C5)=CC=C42)C=6C=CC=CC16 |
| Storage | keep away from direct sunlight | -20°C for 2 years, 4°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||
| Solubility Information | Ethanol: 10 mg/mL (23.62 mM), Sonication is recommended. DMF: 20 mg/mL (47.23 mM), Sonication is recommended. Ethanol:PBS (pH 7.2)(1:3): 0.5 mg/mL (1.18 mM), Sonication is recommended. DMSO: 8 mg/mL (18.89 mM), Sonication is recommended. | |||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||
DMSO/Ethanol/DMF
Ethanol/DMF
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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