Shopping Cart
Remove All
Your shopping cart is currently empty
APF
😃Good
Catalog No. T38099Cas No. 359010-70-1
APF is an aromatic amino-fluorescein derivative that serves as a highly specific fluorescent probe for detecting highly reactive radicals, exhibiting low intrinsic fluorescence but converting into strongly fluorescent fluorescein upon oxidation by hydroxyl radicals, hypochlorite ions, and selected peroxidase intermediates. APF remain unreactive to nitric oxide and hydrogen peroxide, while with fluorescein displays excitation and emission maxima of 490 nm and 515 nm, respectively, enabling sensitive visualization of oxidative stress in biological systems.
APF
😃Good
Catalog No. T38099Cas No. 359010-70-1
APF is an aromatic amino-fluorescein derivative that serves as a highly specific fluorescent probe for detecting highly reactive radicals, exhibiting low intrinsic fluorescence but converting into strongly fluorescent fluorescein upon oxidation by hydroxyl radicals, hypochlorite ions, and selected peroxidase intermediates. APF remain unreactive to nitric oxide and hydrogen peroxide, while with fluorescein displays excitation and emission maxima of 490 nm and 515 nm, respectively, enabling sensitive visualization of oxidative stress in biological systems.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 500 μg | $204 | 35 days | 35 days | |
| 1 mg | $326 | 35 days | 35 days | |
| 5 mg | Inquiry | 35 days | 35 days |
Add to Cart
Add to Quotation
In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Resource Download
Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | APF is an aromatic amino-fluorescein derivative that serves as a highly specific fluorescent probe for detecting highly reactive radicals, exhibiting low intrinsic fluorescence but converting into strongly fluorescent fluorescein upon oxidation by hydroxyl radicals, hypochlorite ions, and selected peroxidase intermediates. APF remain unreactive to nitric oxide and hydrogen peroxide, while with fluorescein displays excitation and emission maxima of 490 nm and 515 nm, respectively, enabling sensitive visualization of oxidative stress in biological systems. |
| In vitro | In cellular assays for detecting Reactive Oxygen Species (ROS), APF was utilized at a working concentration of 1-10 μM. The probe effectively stained both suspension and adherent cells when incubated at 37°C for 20-60 minutes in serum-free media or PBS. This staining allowed for subsequent detection via fluorescence microscopy or flow cytometry [1]. |
| Molecular Weight | 423.42 |
| Formula | C26H17NO5 |
| Cas No. | 359010-70-1 |
| Smiles | O=C1OC2(C3=CC=C(O)C=C3OC4=CC(OC5=CC=C(N)C=C5)=CC=C42)C=6C=CC=CC16 |
| Storage | keep away from direct sunlight | -20°C for 2 years, 4°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||
| Solubility Information | Ethanol: 10 mg/mL (23.62 mM), Sonication is recommended. DMF: 20 mg/mL (47.23 mM), Sonication is recommended. Ethanol:PBS (pH 7.2)(1:3): 0.5 mg/mL (1.18 mM), Sonication is recommended. DMSO: 8 mg/mL (18.89 mM), Sonication is recommended. | |||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||
DMSO/Ethanol/DMF
Ethanol/DMF
| ||||||||||||||||||||||||||
Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . A total of 10 animals were administered, and the formula you used is 5% 
DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。Stock solution preparation method: 2 mg of drug dissolved in 50 μL DMSO (stock solution concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
(stock solution concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.Preparation method for in vivo formula: Take 50 μL DMSO main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarify
main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarifyThe above example illustrates how to use "In Vivo Formulation Calculator" and does not represent a recommended formulation for any specific compound. Please select an appropriate dissolution method based on your experimental animals and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Keywords
Preview
Related Tags: buy APF | purchase APF | APF cost | order APF | APF chemical structure | APF in vitro | APF formula | APF molecular weight
Generate Quote
Catalog No.: Cas No.:
Add
| Size | Quantity | Unit Price | Amount | Operation |
|---|
Generate Quote
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2026 TargetMol Chemicals Inc. All Rights Reserved.