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NanoBRET 590 C4 SE
Catalog No. T212883 Copy Product Info
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NanoBRET 590 C4 SE is an amine-reactive energy acceptor dye specifically designed for NanoBRET assays, emitting orange-red to red fluorescence. It has an emission maximum at approximately 590 nm (detection range: 590–610 nm) and exhibits good spectral overlap with the donor emission of NanoLuc luciferase (~460 nm), enabling efficient bioluminescence resonance energy transfer (BRET). The dye contains an N-hydroxysuccinimide (NHS) ester group, which reacts with primary amines to form stable amide bonds. Its linker incorporates a cyclobutyl-like structural feature, providing unique spatial configuration for tracer design.
NanoBRET 590 C4 SE
Copy Product Info😃Good
Catalog No. T212883
NanoBRET 590 C4 SE is an amine-reactive energy acceptor dye specifically designed for NanoBRET assays, emitting orange-red to red fluorescence. It has an emission maximum at approximately 590 nm (detection range: 590–610 nm) and exhibits good spectral overlap with the donor emission of NanoLuc luciferase (~460 nm), enabling efficient bioluminescence resonance energy transfer (BRET). The dye contains an N-hydroxysuccinimide (NHS) ester group, which reacts with primary amines to form stable amide bonds. Its linker incorporates a cyclobutyl-like structural feature, providing unique spatial configuration for tracer design.
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| Description | NanoBRET 590 C4 SE is an amine-reactive energy acceptor dye specifically designed for NanoBRET assays, emitting orange-red to red fluorescence. It has an emission maximum at approximately 590 nm (detection range: 590–610 nm) and exhibits good spectral overlap with the donor emission of NanoLuc luciferase (~460 nm), enabling efficient bioluminescence resonance energy transfer (BRET). The dye contains an N-hydroxysuccinimide (NHS) ester group, which reacts with primary amines to form stable amide bonds. Its linker incorporates a cyclobutyl-like structural feature, providing unique spatial configuration for tracer design. |
| Molecular Weight | 511.29 |
| Formula | C24H24BF2N5O5 |
| Smiles | O=C(CCCNC(CCC1=CC=C2C=C3C=CC(C4=CC=CN4)=[N+]3[B-](F)(N21)F)=O)ON5C(CCC5=O)=O |
| Storage | Keep away from direct sunlight | Store at -20°C | Shipping with blue ice/Shipping at ambient temperature. |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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