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Lucifer Yellow CH dipotassium

Name: Lucifer Yellow CH dipotassium
Brand: TargetMol
SKU: T78454
Rating: 4.5 (1 reviews)

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Catalog No. T78454Cas No. 71206-95-6

Lucifer Yellow CH dipotassium is a water-soluble fluorescent tracer that labels endocytosis vesicles in cells.Lucifer Yellow CH dipotassium excites and emits at a maximum wavelength of 430 nm and 540 nm, respectively.

Lucifer Yellow CH dipotassium

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Purity: 99.71%

Catalog No. T78454Cas No. 71206-95-6

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
25 mg	Inquiry	In Stock	In Stock
50 mg	Inquiry	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products. If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

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Batch Information

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Purity:99.71%

Appearance:Solid

Color:Orange

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description

In vitro

Lysosome Labeling Protocol[3]
Step 1: Dye Uptake
Seed Thio-macrophages or J774.2 cells onto coverslips.
Incubate cells in medium containing Lucifer Yellow CH (1 mg/ml) for 1-2 hours.

Step 2: Washing
Wash the cells with ice-cold PD buffer (137 mM NaCl/3 mM KCl/7 mM phosphate, pH 7.4).

Step 3: Dye Efflux
Transfer the cells to M1OF medium without Lucifer Yellow CH and continue incubation for 20-30 minutes at 37°C to allow dye efflux from the pinosomal/endosomal compartment.

Step 4: Microscopic Observation
Invert the coverslip onto a microscope slide with M1OF (37°C) as the cell medium.
Observe live cells using a Zeiss light microscope equipped with a 63x oil immersion lens (n.a. 1.4), and capture images with Kodak Tri-X film (ASA 400).

Notes:
This protocol is based on the reference literature. According to other sources and depending on the experimental goals, the working concentration of Lucifer Yellow CH may range from 0.05 to 1 mg/mL. Please adjust the concentration based on the specific requirements of your experiment.

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.

Chemical Properties

Molecular Weight	521.57
Formula	C₁₃H₉K₂N₅O₉S₂
Cas No.	71206-95-6
Smiles	O=C(NN1C(C2=CC(S(=O)([O-])=O)=CC3=C(C(S(=O)([O-])=O)=CC(C1=O)=C23)N)=O)NN.[K+].[K+]

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 4 mg/mL (7.67 mM), Sonication is recommended.

DMSO: 60 mg/mL (115.04 mM), Sonication is recommended.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.9173 mL	9.5864 mL	19.1729 mL	95.8644 mL
5 mM	0.3835 mL	1.9173 mL	3.8346 mL	19.1729 mL

DMSO

	1mg	5mg	10mg	50mg
10 mM	0.1917 mL	0.9586 mL	1.9173 mL	9.5864 mL
20 mM	0.0959 mL	0.4793 mL	0.9586 mL	4.7932 mL
50 mM	0.0383 mL	0.1917 mL	0.3835 mL	1.9173 mL
100 mM	0.0192 mL	0.0959 mL	0.1917 mL	0.9586 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc