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Lucifer Yellow CH dipotassium salt

Catalog No. T78454 CAS 71206-95-6

Lucifer Yellow CH dipotassium, a high-intensity fluorescent probe featuring free hydrazyl groups, reacts with fatty aldehydes at room temperature and functions as a biological tracer for neuronal branching, regeneration, gap junction detection, and characterization, as well as selective cell ablation following aldehyde fixation. It exhibits peak excitation and emission wavelengths of 430 nm and 540 nm, respectively [1] [2].

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Lucifer Yellow CH dipotassium salt Chemical Structure

Lucifer Yellow CH dipotassium salt, CAS 71206-95-6

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2 mg	5 days	$ 155.00

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Biological Description

Chemical Properties

Storage & Solubility Information

Description

In vitro

Prepare a stock solution of Lucifer Yellow CH (dipotassium) by diluting to 1 mg/mL with DDH2O, e.g., dissolve 1 mg of the compound in 1 mL of DDH2O. The solution should be aliquoted and stored away from light at -20℃ or -80℃. For the working solution, dilute the stock solution with pre-warmed serum-free cell culture medium or PBS to achieve a concentration of 0.5-1% Lucifer Yellow CH (dipotassium), adjusting the concentration as necessary for specific applications and prepare fresh before use. For cell staining, spin down suspended cells, washing them twice with PBS for 5 minutes each time. For adherent cells, remove the culture medium, treat with trypsin to detach, then centrifuge and wash twice with PBS. Incubate cells with 1 mL of Lucifer Yellow CH (dipotassium) working solution at room temperature for 5-30 minutes. Centrifuge at 400 g for 3-4 minutes at 4℃, discard the supernatant, and wash the cells twice with PBS. Resuspend the cells in 1 mL of serum-free culture medium or PBS for analysis via fluorescence microscopy or flow cytometry. Note that the stock solution should be protected from repeated freeze-thaw cycles, with a -20℃ shelf life of one month and -80℃ for six months. Adjust working solution concentrations to meet experimental requirements. This product is for research use by professionals only, and not for clinical diagnostics or therapeutic procedures, food, or drug use. Ensure proper safety by wearing lab attire and disposable gloves during handling.

Molecular Weight	521.57
Formula	C13H9K2N5O9S2
CAS No.	71206-95-6

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keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

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Lucifer Yellow CH dipotassium salt 71206-95-6 inhibitor inhibit

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