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PFB-FDGlu

Name: PFB-FDGlu
Brand: TargetMol
SKU: T39518
Price: 152 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T39518 Copy Product Info

Purity: 99.86%

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PFB-FDGlu belongs to fluorescent dyes and is a lysosomal β-glucocerebrosidase (GCase) substrate that releases fluorescein upon hydrolysis by GCase, possessing cell permeability. This compound can be used for flow cytometric detection of GCase activity in live cells.

Cas No. 209540-62-5

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Pack Size	Price	USA Stock	Global Stock
1 mg	$152	In Stock	In Stock
5 mg	$373	In Stock	In Stock
10 mg	$556	In Stock	In Stock
25 mg	$896	-	In Stock
50 mg	$1,220	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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Custom-made molecule. TargetMol’s expert synthesis team delivers cost-effective solutions. Contact us for inquiries. Committed to serving you.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.86%

ee:100%

Appearance:Solid

Color:Yellow

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COA CSFC FNMR ORP LCMS HNMR

Product Introduction

Bioactivity

Description

In vitro

Methods :Monocytes were isolated from human PBMCs and incubated with 0.75 mM PFB-FDGlu for 30 minutes, with a CBE inhibition control group included. Fluorescence in the FL-1 channel was detected by flow cytometry.
Results: PFB-FDGlu was specifically hydrolyzed by intracellular GCase, and its fluorescent signal could be completely inhibited by CBE, indicating that this probe can effectively and specifically detect GCase activity in living cells.[1]
Methods: Human iPSC-derived neural stem cells, neurons, SH-SY5Y cells, and GBA1 knockout neural cells were treated with 75 nM PFB-FDGlu and 20 nM Hoechst 33342, followed by live-cell imaging detection after 45 minutes.
Results: PFB-FDGlu fluorescent signals colocalized with lysosomes and were dose-dependently blocked by the GCase inhibitor CBE. Signals were significantly reduced in GBA1 knockout cells, demonstrating specific reflection of lysosomal GCase activity.[2]

Chemical Properties

Molecular Weight	865.66
Formula	C₃₉H₃₂F₅NO₁₆
Cas No.	209540-62-5
Smiles	O=C1OC2(C=3C1=CC(NC(=O)C4=C(F)C(F)=C(F)C(F)=C4F)=CC3)C=5C(OC=6C2=CC=C(O[C@H]7[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O7)C6)=CC(O[C@H]8[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O8)=CC5
Relative Density.	1.82 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 252.5 mg/mL (291.68 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.1552 mL	5.7759 mL	11.5519 mL	57.7594 mL
5 mM	0.2310 mL	1.1552 mL	2.3104 mL	11.5519 mL
10 mM	0.1155 mL	0.5776 mL	1.1552 mL	5.7759 mL
20 mM	0.0578 mL	0.2888 mL	0.5776 mL	2.8880 mL
50 mM	0.0231 mL	0.1155 mL	0.2310 mL	1.1552 mL
100 mM	0.0116 mL	0.0578 mL	0.1155 mL	0.5776 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.