Acridine Orange Staining Solution

Acridine Orange is a tricyclic aromatic fluorescent dye with membrane permeability that can penetrate cell membranes and bind to nucleic acid molecules. It is commonly used for detecting DNA and RNA within cells. When acridine orange binds to double stranded DNA, the fluorescence emission wavelength is 530 nm, and it emits green fluorescence upon excitation; When acridine orange binds to single stranded DNA or RNA, the fluorescence emission wavelength is 640 nm, and upon excitation, it emits orange red fluorescence. Acridine orange staining is commonly used for detecting cell apoptosis. In normal cells, acridine orange can penetrate the cell membrane and bind to DNA in the nucleus, exhibiting green or yellow green fluorescence; In apoptotic cells, due to chromatin condensation or breakage, acridine orange results in dense and concentrated yellow green fluorescence or yellow green fragmented particles; The cell membrane permeability of necrotic cells increases, the nucleic acid structure is disrupted, and fluorescence weakens or even disappears.