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Biotin-4-Fluorescein
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Catalog No. T88718Cas No. 1032732-74-3
Biotin-4-Fluorescein is a high-affinity fluorescent tracer probe consisting of biotin covalently linked to a fluorescein molecule. Leveraging the exceptionally strong non-covalent interaction between biotin and avidin or streptavidin, this compound is widely used in competitive binding assays for the quantitative analysis of the binding affinities of biotin and its derivatives. The significant quenching of its fluorescence upon binding to avidin makes it an ideal molecular tool for kinetic studies and high-throughput screening.
Biotin-4-Fluorescein
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Purity: 99.72%
Catalog No. T88718Cas No. 1032732-74-3
Biotin-4-Fluorescein is a high-affinity fluorescent tracer probe consisting of biotin covalently linked to a fluorescein molecule. Leveraging the exceptionally strong non-covalent interaction between biotin and avidin or streptavidin, this compound is widely used in competitive binding assays for the quantitative analysis of the binding affinities of biotin and its derivatives. The significant quenching of its fluorescence upon binding to avidin makes it an ideal molecular tool for kinetic studies and high-throughput screening.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 1 mg | $136 | - | In Stock | |
| 5 mg | $300 | - | In Stock | |
| 10 mg | $450 | - | In Stock | |
| 25 mg | $766 | - | In Stock | |
| 50 mg | $1,140 | - | In Stock |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Purity:99.72%
Appearance:Solid
Color:Orange
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | Biotin-4-Fluorescein is a high-affinity fluorescent tracer probe consisting of biotin covalently linked to a fluorescein molecule. Leveraging the exceptionally strong non-covalent interaction between biotin and avidin or streptavidin, this compound is widely used in competitive binding assays for the quantitative analysis of the binding affinities of biotin and its derivatives. The significant quenching of its fluorescence upon binding to avidin makes it an ideal molecular tool for kinetic studies and high-throughput screening. |
| In vitro | In biochemical assays, Biotin-4-Fluorescein binds to avidin with extremely high affinity, and its fluorescence is significantly quenched upon complex formation, allowing for the precise measurement of dissociation constants and the development of sensitive competitive binding assays [1]. |
| In vivo | In pharmacological studies focused on targeted drug delivery systems, Biotin-4-Fluorescein was utilized as a tracer to evaluate the biodistribution and receptor-mediated uptake of biotinylated carriers in rodent models; the results demonstrated that the probe effectively visualized the accumulation of functionalized nanoparticles in specific tissues through its high-affinity interaction with pre-administered avidin-conjugated targets [1]. |
| Molecular Weight | 644.69 |
| Formula | C33H32N4O8S |
| Cas No. | 1032732-74-3 |
| Smiles | O=C1OC2(C=3C1=CC(C(NCCNC(CCCC[C@H]4[C@@]5([C@](CS4)(NC(=O)N5)[H])[H])=O)=O)=CC3)C=6C(OC=7C2=CC=C(O)C7)=CC(O)=CC6 |
| Storage | keep away from direct sunlight | store at -20°C | Shipping with blue ice/Shipping at ambient temperature. |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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