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O-Propargyl-Puromycin

Name: O-Propargyl-Puromycin
Brand: TargetMol
SKU: T12284
Price: 46 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T12284Cas No. 1416561-90-4

Alias O-Propargylpuromycin, OP-puro

O-Propargyl-Puromycin (OP-puro) is a potent protein synthesis inhibitor, a puromycin acetylene analog.

O-Propargyl-Puromycin

🥰Excellent

Purity: 99.29%

Catalog No. T12284Alias O-Propargylpuromycin, OP-puroCas No. 1416561-90-4

O-Propargyl-Puromycin (OP-puro) is a potent protein synthesis inhibitor, a puromycin acetylene analog.

Pack Size	Price	USA Warehouse	Global Warehouse
500 μg	$46	In Stock	In Stock
1 mg	$66	In Stock	In Stock
5 mg	$158	In Stock	In Stock
10 mg	$248	In Stock	In Stock
25 mg	$397	In Stock	In Stock
50 mg	$569	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$188	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products. If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

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Purity:99.29%

ee:97.20%

Appearance:Solid

Color:White

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COA HNMR LCMS CSFC

Product Introduction

Bioactivity

Description	O-Propargyl-Puromycin (OP-puro) is a potent protein synthesis inhibitor, a puromycin acetylene analog.
In vitro	O-Propargyl-Puromycin can be used to image and affinity purify nascent proteins in cells and animals. O-Propargyl-Puromycin inhibits protein synthesis in reticulocyte lysates and cultured cells with two to three times less potency than unmodified puromycin. O-Propargyl-Puromycin forms covalent conjugates with nascent polypeptide chains that are rapidly turned over by the proteasome and can be visualized or trapped by copper(I)-catalyzed azide-alkyne cycloaddition reactions.[1]
In vivo	Tissue from uninjected mice showed low nonspecific staining, whereas tissue from O-Propargyl-Puromycin-injected mice showed a specific pattern of O-Propargyl-Puromycin incorporation into nascent proteins. In the small intestine, cells in the crypts and at the base of intestinal villi have the greatest translation capacity, consistent with the high proliferative and secretory activity of these cells. Staining was particularly intense in Paneth cells, which are located near the base of the crypts and are filled with secretory vesicles. Intense O-Propargyl-Puromycin labeling of vesicles in Paneth cells suggests that prematurely terminated O-Propargyl-Puromycin-conjugated secretory proteins translocate into the endoplasmic reticulum (ER) lumen.[1]
Synonyms	O-Propargylpuromycin, OP-puro

Chemical Properties

Molecular Weight	495.53
Formula	C₂₄H₂₉N₇O₅
Cas No.	1416561-90-4
Smiles	CN(C)c1ncnc2n(cnc12)[C@@H]1O[C@H](CO)[C@@H](NC(=O)[C@@H](N)Cc2ccc(OCC#C)cc2)[C@H]1O
Relative Density.	1.44 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 257.5 mg/mL (519.65 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (10.09 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0180 mL	10.0902 mL	20.1804 mL	100.9021 mL
5 mM	0.4036 mL	2.0180 mL	4.0361 mL	20.1804 mL
10 mM	0.2018 mL	1.0090 mL	2.0180 mL	10.0902 mL
20 mM	0.1009 mL	0.5045 mL	1.0090 mL	5.0451 mL
50 mM	0.0404 mL	0.2018 mL	0.4036 mL	2.0180 mL
100 mM	0.0202 mL	0.1009 mL	0.2018 mL	1.0090 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc