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Evans blue

Name: Evans blue
Brand: TargetMol
SKU: T0313
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: Direct Blue 53, C.I. 23860) Copy Product Info

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Synonyms: Direct Blue 53, C.I. 23860

Catalog No. T0313 Copy Product Info

Purity: 97.13%

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Evans blue (C.I. 23860) is an effective inhibitor of glutamate uptake by synaptic vesicles and also acts as an antagonist of AMPA/kainate receptors. Evans blue is a water-soluble azo fluorescent dye that binds to serum albumin and is used to detect vascular permeability, blood-brain barrier integrity, and permeability.

Cas No. 314-13-6

Pack Size	Price	USA Stock	Global Stock
1 g	$38	-	In Stock
5 g	$86	-	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:97.13%

Color:Brown to Black

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	Evans blue (C.I. 23860) is an effective inhibitor of glutamate uptake by synaptic vesicles and also acts as an antagonist of AMPA/kainate receptors. Evans blue is a water-soluble azo fluorescent dye that binds to serum albumin and is used to detect vascular permeability, blood-brain barrier integrity, and permeability.
Targets & IC50	AMPA receptor subtype:355 nM, CaMK IIa:197.1 nM, Glutamate uptake (vesicular):87 nM, AMPA:220 nM, kainate receptor:150 nM
In vitro	Methods: Human glioblastoma U87MG cells (FAP-positive) were treated with ¹⁷⁷Lu-Evans blue-FAPI-B1, B2, B3, B4 (37 kBq) tracers added to each well. Blocking group additionally received 10 μg/well FAPI-02. Treated for 0.5, 1, 2, 4, 8, 24 h. Radioactivity measured by γ-counter to calculate cellular uptake rate (%). Results: All tracers showed time-dependent uptake peaking at 24 h. Blocking agent reduced uptake rate, confirming binding specificity. [1] Methods: HT22 mouse hippocampal neuronal cells were preincubated with Evans blue (1 mM) for 2 hours, then stimulated with glutamate (100 μM) for 30 minutes. ATP concentration in cell culture supernatant was measured by bioluminescence assay. Results: Glutamate stimulation increased ATP release in HT22 cells, and Evans blue pretreatment completely blocked this effect. [2]
In vivo	Methods: U87MG tumor-bearing nude mice (initial tumor size ~100 mm³) received a single tail vein injection of different doses of the radiotracer ¹⁷⁷Lu-Evans blue-FAPI-B1 (7.4, 18.5, 30 MBq/mouse). Tumors were observed for 22 days post-treatment. Results: All treatment groups showed significant inhibition of tumor growth compared to the saline control group. [1] Methods: Male SD rats underwent L5 spinal nerve ligation (SNL) model. Intrathecal Evans blue (50 µg/rat) was administered as a single dose starting 7 days post-surgery, with observations conducted for 7 days. Results: Evans blue increased the mechanical pain threshold in a dose-dependent manner, with 50 µg showing optimal efficacy. Analgesic effects persisted for approximately 5 days.[2]
Synonyms	Direct Blue 53, C.I. 23860

Chemical Properties

Molecular Weight	960.78
Formula	C₃₄H₂₄N₆Na₄O₁₄S₄
Cas No.	314-13-6
Smiles	[Na+].[Na+].[Na+].[Na+].CC1=CC(=CC=C1NN=C1C=CC2=C(C(N)=C(C=C2S([O-])(=O)=O)S([O-])(=O)=O)C1=O)C1=CC(C)=C(NN=C2C=CC3=C(C(N)=C(C=C3S([O-])(=O)=O)S([O-])(=O)=O)C2=O)C=C1
Relative Density.	no data available

Storage & Solubility Information

Storage

Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 104.1 mM, Sonication is recommended.

DMSO: 104.1 mM, Sonication is recommended.

In Vivo Formulation

PBS: 100 mg/mL (104.08 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.0408 mL	5.2041 mL	10.4082 mL	52.0410 mL
5 mM	0.2082 mL	1.0408 mL	2.0816 mL	10.4082 mL
10 mM	0.1041 mL	0.5204 mL	1.0408 mL	5.2041 mL
20 mM	0.0520 mL	0.2602 mL	0.5204 mL	2.6021 mL
50 mM	0.0208 mL	0.1041 mL	0.2082 mL	1.0408 mL
100 mM	0.0104 mL	0.0520 mL	0.1041 mL	0.5204 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc