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Evans blue

Name: Evans blue
Brand: TargetMol
SKU: T0313
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T0313Cas No. 314-13-6

Alias Direct Blue 53, C.I. 23860

Evans blue (C.I. 23860) is an effective inhibitor of glutamate uptake by synaptic vesicles and also acts as an antagonist of AMPA/kainate receptors. As an azo dye, Evans blue binds to serum albumin, enabling detection of blood-brain barrier integrity and permeability.

Evans blue

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Purity: 97.13%

Catalog No. T0313Alias Direct Blue 53, C.I. 23860Cas No. 314-13-6

Pack Size	Price	USA Warehouse	Global Warehouse
1 g	$38	-	In Stock
5 g	$86	-	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:97.13%

Color:Brown to Black

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	Evans blue (C.I. 23860) is an effective inhibitor of glutamate uptake by synaptic vesicles and also acts as an antagonist of AMPA/kainate receptors. As an azo dye, Evans blue binds to serum albumin, enabling detection of blood-brain barrier integrity and permeability.
Targets&IC50	AMPA:220 nM, kainate receptor:150 nM, Glutamate uptake (vesicular):87 nM, CaMK IIa:197.1 nM, AMPA receptor subtype:355 nM
In vitro	Evans Blue is found to be inhibiting DNA binding of NF-κB at a low concentration of 100 μM[1]. It has proven over the years to be a dependable stain for microscopic determination of cell death[2]. Evans Blue is also a known blocker of a subset of a-amino-3-hydroxy-5-methyl-isoxazole/kainate receptors (IC50=355 nM) for the subunit combination GluR1,2[5]. Evans blue is the first known δ-subunit-specific antagonist of ENaC and activates large-conductance Ca2+-activated K+ channels in sheep bladder myocytes and cultured endothelial cells of human umbilical veins[5].
In vivo	Evans blue pretreatment could inhibit the mast cells degranulation and that may be the main mechanism for prevent animals from C48/80-triggered anaphylaxis and systemic inflammation[4]. Evans blue modulates the non-N-methyl-d-aspartate receptor in rat thalamic neurons, blocks the P2X-purinergic receptor in rat vas deferens, and inhibits the glutamate transporter in rat brain synaptic vesicles[6].
Synonyms	Direct Blue 53, C.I. 23860

Chemical Properties

Molecular Weight	960.78
Formula	C₃₄H₂₄N₆Na₄O₁₄S₄
Cas No.	314-13-6
Smiles	[Na+].[Na+].[Na+].[Na+].CC1=CC(=CC=C1NN=C1C=CC2=C(C(N)=C(C=C2S([O-])(=O)=O)S([O-])(=O)=O)C1=O)C1=CC(C)=C(NN=C2C=CC3=C(C(N)=C(C=C3S([O-])(=O)=O)S([O-])(=O)=O)C2=O)C=C1
Relative Density.	no data available

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 104.1 mM, Sonication is recommended.

DMSO: 104.1 mM, Sonication is recommended.

In Vivo Formulation

PBS: 100 mg/mL (104.08 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.0408 mL	5.2041 mL	10.4082 mL	52.0410 mL
5 mM	0.2082 mL	1.0408 mL	2.0816 mL	10.4082 mL
10 mM	0.1041 mL	0.5204 mL	1.0408 mL	5.2041 mL
20 mM	0.0520 mL	0.2602 mL	0.5204 mL	2.6021 mL
50 mM	0.0208 mL	0.1041 mL	0.2082 mL	1.0408 mL
100 mM	0.0104 mL	0.0520 mL	0.1041 mL	0.5204 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc