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Evans blue

Name: Evans blue
Brand: TargetMol
SKU: T0313
Price: 37 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T0313Cas No. 314-13-6

Alias Direct Blue 53, C.I. 23860

Evans blue (C.I. 23860) is an azo dye, inhibits L-glutamate uptake via the membrane-bound excitatory amino acid transporter (EAAT).

Evans blue

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Purity: 97.13%

Catalog No. T0313Alias Direct Blue 53, C.I. 23860Cas No. 314-13-6

Evans blue (C.I. 23860) is an azo dye, inhibits L-glutamate uptake via the membrane-bound excitatory amino acid transporter (EAAT).

Pack Size	Price	Availability
50 mg	$37	In Stock
100 mg	$50	In Stock
500 mg	$89	In Stock
1 g	$108	In Stock
5 g	$179	In Stock
1 mL x 10 mM (in DMSO)	$57	In Stock

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Purity:97.13%

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COA HNMR

Product Introduction

Bioactivity

Description	Evans blue (C.I. 23860) is an azo dye, inhibits L-glutamate uptake via the membrane-bound excitatory amino acid transporter (EAAT).
Targets&IC50	kainate receptor:150 nM, Glutamate uptake (vesicular):87 nM, AMPA receptor subtype:355 nM, AMPA:220 nM, CaMK IIa:197.1 nM
In vitro	Evans Blue is found to be inhibiting DNA binding of NF-κB at a low concentration of 100 μM[1]. It has proven over the years to be a dependable stain for microscopic determination of cell death[2]. Evans Blue is also a known blocker of a subset of a-amino-3-hydroxy-5-methyl-isoxazole/kainate receptors (IC50=355 nM) for the subunit combination GluR1,2[5]. Evans blue is the first known δ-subunit-specific antagonist of ENaC and activates large-conductance Ca2+-activated K+ channels in sheep bladder myocytes and cultured endothelial cells of human umbilical veins[5].
In vivo	Evans blue pretreatment could inhibit the mast cells degranulation and that may be the main mechanism for prevent animals from C48/80-triggered anaphylaxis and systemic inflammation[4]. Evans blue modulates the non-N-methyl-d-aspartate receptor in rat thalamic neurons, blocks the P2X-purinergic receptor in rat vas deferens, and inhibits the glutamate transporter in rat brain synaptic vesicles[6].
Synonyms	Direct Blue 53, C.I. 23860

Chemical Properties

Molecular Weight	960.78
Formula	C₃₄H₂₄N₆Na₄O₁₄S₄
Cas No.	314-13-6
Smiles	[Na+].[Na+].[Na+].[Na+].CC1=CC(=CC=C1NN=C1C=CC2=C(C(N)=C(C=C2S([O-])(=O)=O)S([O-])(=O)=O)C1=O)C1=CC(C)=C(NN=C2C=CC3=C(C(N)=C(C=C3S([O-])(=O)=O)S([O-])(=O)=O)C2=O)C=C1
Relative Density.	no data available

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 104.1 mM, Sonication is recommended.

DMSO: 104.1 mM, Sonication is recommended.

In Vivo Formulation

PBS: 100 mg/mL (104.08 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.0408 mL	5.2041 mL	10.4082 mL	52.0410 mL
5 mM	0.2082 mL	1.0408 mL	2.0816 mL	10.4082 mL
10 mM	0.1041 mL	0.5204 mL	1.0408 mL	5.2041 mL
20 mM	0.0520 mL	0.2602 mL	0.5204 mL	2.6021 mL
50 mM	0.0208 mL	0.1041 mL	0.2082 mL	1.0408 mL
100 mM	0.0104 mL	0.0520 mL	0.1041 mL	0.5204 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc