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FITC-Dextran (MW 110000) is a compound classified as a fluorescent dye. It is commonly used in biomedical research as a tracer molecule to label and track cells or other biological materials. Composed of fluorescein isothiocyanate (FITC) and dextran, where dextran is a complex carbohydrate derived from starch, this combination forms a stable fluorescent tracer that can be observed under a microscope or quantified using specialized detection instruments.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 10 mg | Inquiry | Inquiry | Inquiry | |
| 50 mg | Inquiry | Inquiry | Inquiry |
| Description | FITC-Dextran (MW 110000) is a compound classified as a fluorescent dye. It is commonly used in biomedical research as a tracer molecule to label and track cells or other biological materials. Composed of fluorescein isothiocyanate (FITC) and dextran, where dextran is a complex carbohydrate derived from starch, this combination forms a stable fluorescent tracer that can be observed under a microscope or quantified using specialized detection instruments. |
| In vitro | FITC-Dextran (MW 110000) is a fluorescein isothiocyanate (FITC) dextran fluorescent probe with excitation and emission wavelengths of 495 nm and 525 nm, respectively. It serves as a tracer to reveal cell damage induced by heat shock and to study both early and late stages of apoptosis. Additionally, FITC-Dextran (MW 110000) is used in research on cell permeability, including the assessment of blood-brain barrier permeability and disruption. Store it protected from light. |
| In vivo | Assessment of Intestinal Barrier Function: 1. Subject mice to a fasting period of 4 hours. 2. Administer FITC-Dextran with a molecular weight of 110,000 to the mice via gavage (0.6 mg/g). 3. Measure the fluorescence intensity (Ex nm/Em 520 nm) within 4 hours. |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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