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Boc-Leu-Gly-Arg-AMC

🥰Excellent
Catalog No. TP2169Cas No. 65147-09-3

Boc-Leu-Gly-Arg-AMC is a specific fluorogenic substrate designed for the enzymatic detection and quantification of complement component C3/C5 convertases, coagulation factor Xa, and soybean trypsin-like enzyme activity. Boc-Leu-Gly-Arg-AMC is also susceptible to hydrolysis by macropain, a high molecular weight thiol proteinase isolated from human erythrocytes, making it a versatile tool for enzymatic assays.

Boc-Leu-Gly-Arg-AMC

Boc-Leu-Gly-Arg-AMC

🥰Excellent
Purity: 99.53%
Catalog No. TP2169Cas No. 65147-09-3
Boc-Leu-Gly-Arg-AMC is a specific fluorogenic substrate designed for the enzymatic detection and quantification of complement component C3/C5 convertases, coagulation factor Xa, and soybean trypsin-like enzyme activity. Boc-Leu-Gly-Arg-AMC is also susceptible to hydrolysis by macropain, a high molecular weight thiol proteinase isolated from human erythrocytes, making it a versatile tool for enzymatic assays.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
1 mg$44-In Stock
5 mg$98-In Stock
10 mg$158-In Stock
25 mg$272-In Stock
50 mg$413-In Stock
100 mg$589-In Stock
200 mg$839-In Stock
1 mL x 10 mM (in DMSO)$147-In Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Purity:99.53%
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COA HPLC MS

Product Introduction

Bioactivity
Description
Boc-Leu-Gly-Arg-AMC is a specific fluorogenic substrate designed for the enzymatic detection and quantification of complement component C3/C5 convertases, coagulation factor Xa, and soybean trypsin-like enzyme activity. Boc-Leu-Gly-Arg-AMC is also susceptible to hydrolysis by macropain, a high molecular weight thiol proteinase isolated from human erythrocytes, making it a versatile tool for enzymatic assays.
In vitro
To demonstrate the presence of proteolytic enzymes capable of producing sodifrin in the abdominal gland, polybutoxycarbo-nyl (Boc)-Leu-Gly-Arg-4-methylcoumaryl-7-amide (MCA) and Boc-Leu-Leu-Lys-MCA were used as synthetic substrates. Crude extracts of the abdominal gland hydrolyzed both substrates to release 7-amino-4-methylcoumarin, indicating the presence of enzymes in the gland that generate sodifrin from its precursor molecule. The activity of the extracts to cleave Boc-Leu-Gly-Arg-AMC was optimal at pH 9.0 and 45°C, while the activity to cleave Boc-Leu-Leu-Lys-MCA was optimal at pH 9.0 and 40°C. [1]
Chemical Properties
Molecular Weight601.69
FormulaC29H43N7O7
Cas No.65147-09-3
SmilesCC=1C=2C(=CC(NC([C@@H](NC(CNC([C@@H](NC(OC(C)(C)C)=O)CC(C)C)=O)=O)CCCNC(=N)N)=O)=CC2)OC(=O)C1
Relative Density.1.31g/cm3
SequenceBoc-Leu-Gly-Arg-AMC
Sequence ShortBoc-LGR-AMC
Storage & Solubility Information
Storagekeep away from moisture,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
DMSO: 80 mg/mL (132.96 mM), Sonication is recommended.
H2O: < 0.1 mg/mL (insoluble), Sonication is recommended.
In Vivo Formulation
10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (5.48 mM), Sonication is recommended.
Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM1.6620 mL8.3099 mL16.6199 mL83.0993 mL
5 mM0.3324 mL1.6620 mL3.3240 mL16.6199 mL
10 mM0.1662 mL0.8310 mL1.6620 mL8.3099 mL
20 mM0.0831 mL0.4155 mL0.8310 mL4.1550 mL
50 mM0.0332 mL0.1662 mL0.3324 mL1.6620 mL
100 mM0.0166 mL0.0831 mL0.1662 mL0.8310 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.
1 Enter information below:
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2 Enter the in vivo formulation:
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Keywords

Boc-Leu-Gly-Arg-AMCBocLeuGlyArgAMCBoc Leu Gly Arg AMC
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