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Boc-Leu-Gly-Arg-AMC

Name: Boc-Leu-Gly-Arg-AMC
Brand: TargetMol
SKU: TP2169
Price: 44 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. TP2169Cas No. 65147-09-3

Boc-Leu-Gly-Arg-AMC is a specific fluorogenic substrate designed for the enzymatic detection and quantification of complement component C3/C5 convertases, coagulation factor Xa, and soybean trypsin-like enzyme activity. Boc-Leu-Gly-Arg-AMC is also susceptible to hydrolysis by macropain, a high molecular weight thiol proteinase isolated from human erythrocytes, making it a versatile tool for enzymatic assays.

Boc-Leu-Gly-Arg-AMC

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Purity: 99.53%

Catalog No. TP2169Cas No. 65147-09-3

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$44	-	In Stock
5 mg	$98	-	In Stock
10 mg	$158	-	In Stock
25 mg	$272	-	In Stock
50 mg	$413	-	In Stock
100 mg	$589	-	In Stock
200 mg	$839	-	In Stock
1 mL x 10 mM (in DMSO)	$147	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.53%

Appearance:Solid

Color:White

Resource Download

COA HPLC MS

Product Introduction

Bioactivity

Description

In vitro

To demonstrate the presence of proteolytic enzymes capable of producing sodifrin in the abdominal gland, polybutoxycarbo-nyl (Boc)-Leu-Gly-Arg-4-methylcoumaryl-7-amide (MCA) and Boc-Leu-Leu-Lys-MCA were used as synthetic substrates. Crude extracts of the abdominal gland hydrolyzed both substrates to release 7-amino-4-methylcoumarin, indicating the presence of enzymes in the gland that generate sodifrin from its precursor molecule. The activity of the extracts to cleave Boc-Leu-Gly-Arg-AMC was optimal at pH 9.0 and 45°C, while the activity to cleave Boc-Leu-Leu-Lys-MCA was optimal at pH 9.0 and 40°C. [1]

Chemical Properties

Molecular Weight	601.69
Formula	C₂₉H₄₃N₇O₇
Cas No.	65147-09-3
Smiles	CC=1C=2C(=CC(NC([C@@H](NC(CNC([C@@H](NC(OC(C)(C)C)=O)CC(C)C)=O)=O)CCCNC(=N)N)=O)=CC2)OC(=O)C1
Relative Density.	1.31g/cm3
Sequence	Boc-Leu-Gly-Arg-AMC
Sequence Short	Boc-LGR-AMC

Storage & Solubility Information

Storage

keep away from moisture,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (132.96 mM), Sonication is recommended.

H2O: < 0.1 mg/mL (insoluble), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (5.48 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.6620 mL	8.3099 mL	16.6199 mL	83.0993 mL
5 mM	0.3324 mL	1.6620 mL	3.3240 mL	16.6199 mL
10 mM	0.1662 mL	0.8310 mL	1.6620 mL	8.3099 mL
20 mM	0.0831 mL	0.4155 mL	0.8310 mL	4.1550 mL
50 mM	0.0332 mL	0.1662 mL	0.3324 mL	1.6620 mL
100 mM	0.0166 mL	0.0831 mL	0.1662 mL	0.8310 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc